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[KO Validated] IMPDH2 Rabbit mAb (A9208)

KO/KDValidated

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] IMPDH2 Rabbit mAb (A9208)

Western blot analysis of various lysates, using [KO Validated] IMPDH2 Rabbit mAb (A9208) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Western blot - [KO Validated] IMPDH2 Rabbit mAb (A9208)

Western blot analysis of lysates from wild type(WT) and IMPDH2 knockout (KO) NIH/3T3(KO) cells, using [KO Validated] IMPDH2 Rabbit mAb (A9208) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunofluorescence - [KO Validated] IMPDH2 Rabbit mAb (A9208)

Confocal imaging of NIH/3T3 cells using [KO Validated] IMPDH2 Rabbit mAb (A9208, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - [KO Validated] IMPDH2 Rabbit mAb (A9208)

Confocal imaging of C6 cells using [KO Validated] IMPDH2 Rabbit mAb (A9208, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - [KO Validated] IMPDH2 Rabbit mAb (A9208)

Confocal imaging of C2C12 cells using [KO Validated] IMPDH2 Rabbit mAb (A9208, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Overview

Product name[KO Validated] IMPDH2 Rabbit mAb
Catalog No.A9208
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1479
This gene encodes the rate-limiting enzyme in the de novo guanine nucleotide biosynthesis. It is thus involved in maintaining cellular guanine deoxy- and ribonucleotide pools needed for DNA and RNA synthesis. The encoded protein catalyzes the NAD-dependent oxidation of inosine-5'-monophosphate into xanthine-5'-monophosphate, which is then converted into guanosine-5'-monophosphate. This gene is up-regulated in some neoplasms, suggesting it may play a role in malignant transformation.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 100-300 of human IMPDH2 (P12268).
SequenceQANEVRKVKKYEQGFITDPVVLSPKDRVRDVFEAKARHGFCGIPITDTGRMGSRLVGIISSRDIDFLKEEEHDCFLEEIMTKREDLVVAPAGITLKEANEILQRSKKGKLPIVNEDDELVAIIARTDLKKNRDYPLASKDAKKQLLCGAAIGTHEDDKYRLDLLAQAGVDVVVLDSSQGNSIFQINMIKYIKDKYPNLQVI
Gene ID
Swiss Prot
SynonymsIMPD2; IMPDH-II; H2
Calculated MW56kDa
Observed MW56kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHeLa, K-562
Cellular locationCytoplasm, Nucleus

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - [KO Validated] IMPDH2 Rabbit mAb (A9208)}

    Western blot - [KO Validated] IMPDH2 Rabbit mAb (A9208)

    Western blot analysis of various lysates, using [KO Validated] IMPDH2 Rabbit mAb (A9208) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 3s.
    ABclonal:Western blot - [KO Validated] IMPDH2 Rabbit mAb (A9208)}

    Western blot - [KO Validated] IMPDH2 Rabbit mAb (A9208)

    Western blot analysis of lysates from wild type(WT) and IMPDH2 knockout (KO) NIH/3T3(KO) cells, using [KO Validated] IMPDH2 Rabbit mAb (A9208) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 3s.
    ABclonal:Immunofluorescence - [KO Validated] IMPDH2 Rabbit mAb (A9208)}

    Immunofluorescence - [KO Validated] IMPDH2 Rabbit mAb (A9208)

    Confocal imaging of NIH/3T3 cells using [KO Validated] IMPDH2 Rabbit mAb (A9208, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
    ABclonal:Immunofluorescence - [KO Validated] IMPDH2 Rabbit mAb (A9208)}

    Immunofluorescence - [KO Validated] IMPDH2 Rabbit mAb (A9208)

    Confocal imaging of C6 cells using [KO Validated] IMPDH2 Rabbit mAb (A9208, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
    ABclonal:Immunofluorescence - [KO Validated] IMPDH2 Rabbit mAb (A9208)}

    Immunofluorescence - [KO Validated] IMPDH2 Rabbit mAb (A9208)

    Confocal imaging of C2C12 cells using [KO Validated] IMPDH2 Rabbit mAb (A9208, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

    * For research use only. Not for therapeutic or diagnostic purposes.

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