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[KO Validated] KMT5C Rabbit pAb (A16235)

KO/KDValidated

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - [KO Validated] KMT5C Rabbit pAb (A16235)

Western blot analysis of various lysates using [KO Validated] KMT5C Rabbit pAb (A16235) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - [KO Validated] KMT5C Rabbit pAb (A16235)

Western blot analysis of lysates from Mouse thymus, using [KO Validated] KMT5C Rabbit pAb (A16235) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - [KO Validated] KMT5C Rabbit pAb (A16235)

Western blot analysis of lysates from wild type (WT) and KMT5C knockout (KO) 293T cells, using [KO Validated] KMT5C Rabbit pAb (A16235) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product name[KO Validated] KMT5C Rabbit pAb
Catalog No.A16235
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
SUV420H2 and the related enzyme SUV420H1 (MIM 610881) function as histone methyltransferases that specifically trimethylate nucleosomal histone H4 (see MIM 602822) on lysine-20 (K20) (Schotta et al., 2004 [PubMed 15145825]).
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 100-200 of human KMT5C (NP_116090.2).
SequenceFLPESGFTILPCTRYSMETNGAKIVSTRAWKKNEKLELLVGCIAELREADEGLLRAGENDFSIMYSTRKRSAQLWLGPAAFINHDCKPNCKFVPADGNAAC
Gene ID
Swiss Prot
SynonymsSUV420H2; Suv4-20h2; 5C
Calculated MW52kDa
Observed MW52kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, 293T, Jurkat, Mouse thymus
Cellular locationChromosome, Nucleus
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - [KO Validated] KMT5C Rabbit pAb (A16235)}

Western blot - [KO Validated] KMT5C Rabbit pAb (A16235)

Western blot analysis of various lysates using [KO Validated] KMT5C Rabbit pAb (A16235) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - [KO Validated] KMT5C Rabbit pAb (A16235)}

Western blot - [KO Validated] KMT5C Rabbit pAb (A16235)

Western blot analysis of lysates from Mouse thymus, using [KO Validated] KMT5C Rabbit pAb (A16235) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Western blot - [KO Validated] KMT5C Rabbit pAb (A16235)}

Western blot - [KO Validated] KMT5C Rabbit pAb (A16235)

Western blot analysis of lysates from wild type (WT) and KMT5C knockout (KO) 293T cells, using [KO Validated] KMT5C Rabbit pAb (A16235) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (1)

Secondary Antibodies (26)