제품 > 항체 > 다중클론항체(pAb)

[KO Validated] LDHB Rabbit pAb (A18096)

KO/KDValidated

Publication (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] LDHB Rabbit pAb (A18096)

Western blot analysis of lysates from wild type (WT) and LDHB knockout (KO) 293T cells, using [KO Validated] LDHB Rabbit pAb (A18096) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - [KO Validated] LDHB Rabbit pAb (A18096)

Western blot analysis of lysates from wild type(WT) and LDHB knockout (KO) 293T cells, using [KO Validated] LDHB Rabbit pAb (A18096) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.

ABclonal:Immunofluorescence - [KO Validated] LDHB Rabbit pAb (A18096)

Immunofluorescence analysis of Mouse heart tissue using [KO Validated] LDHB Rabbit pAb (A18096) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining.

Overview

Product name[KO Validated] LDHB Rabbit pAb
Catalog No.A18096
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on chromosomes X, 5 and 13.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 2-334 of human LDHB (NP_002291.1).
SequenceATLKEKLIAPVAEEEATVPNNKITVVGVGQVGMACAISILGKSLADELALVDVLEDKLKGEMMDLQHGSLFLQTPKIVADKDYSVTANSKIVVVTAGVRQQEGESRLNLVQRNVNVFKFIIPQIVKYSPDCIIIVVSNPVDILTYVTWKLSGLPKHRVIGSGCNLDSARFRYLMAEKLGIHPSSCHGWILGEHGDSSVAVWSGVNVAGVSLQELNPEMGTDNDSENWKEVHKMVVESAYEVIKLKGYTNWAIGLSVADLIESMLKNLSRIHPVSTMVKGMYGIENEVFLSLPCILNARGLTSVINQKLKDDEVAQLKKSADTLWDIQKDLKDL
Gene ID
Swiss Prot
SynonymsLDH-B; LDH-H; LDHBD; TRG-5; HEL-S-281; HB
Calculated MW37kDa
Observed MW36kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samples293T
Cellular locationCytoplasm.
Customer validation

WB(Rattus norvegicus, Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - [KO Validated] LDHB Rabbit pAb (A18096)}

Western blot - [KO Validated] LDHB Rabbit pAb (A18096)

Western blot analysis of lysates from wild type (WT) and LDHB knockout (KO) 293T cells, using [KO Validated] LDHB Rabbit pAb (A18096) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - [KO Validated] LDHB Rabbit pAb (A18096)}

Western blot - [KO Validated] LDHB Rabbit pAb (A18096)

Western blot analysis of lysates from wild type(WT) and LDHB knockout (KO) 293T cells, using [KO Validated] LDHB Rabbit pAb (A18096) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.
ABclonal:Immunofluorescence - [KO Validated] LDHB Rabbit pAb (A18096)}

Immunofluorescence - [KO Validated] LDHB Rabbit pAb (A18096)

Immunofluorescence analysis of Mouse heart tissue using [KO Validated] LDHB Rabbit pAb (A18096) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A18096? Please let us know so that we can cite the reference in this datasheet.