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[KO Validated] LDHB Rabbit pAb (A18096)

KO/KDValidated

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] LDHB Rabbit pAb (A18096)

Western blot analysis of lysates from wild type (WT) and LDHB knockout (KO) 293T cells, using [KO Validated] LDHB Rabbit pAb (A18096) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - [KO Validated] LDHB Rabbit pAb (A18096)

Western blot analysis of lysates from wild type(WT) and LDHB knockout (KO) 293T cells, using [KO Validated] LDHB Rabbit pAb (A18096) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.

ABclonal:Immunofluorescence - [KO Validated] LDHB Rabbit pAb (A18096)

Immunofluorescence analysis of Mouse heart tissue using [KO Validated] LDHB Rabbit pAb (A18096) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining.

Overview

Product name[KO Validated] LDHB Rabbit pAb
Catalog No.A18096
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on chromosomes X, 5 and 13.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 2-334 of human LDHB (NP_002291.1).
SequenceATLKEKLIAPVAEEEATVPNNKITVVGVGQVGMACAISILGKSLADELALVDVLEDKLKGEMMDLQHGSLFLQTPKIVADKDYSVTANSKIVVVTAGVRQQEGESRLNLVQRNVNVFKFIIPQIVKYSPDCIIIVVSNPVDILTYVTWKLSGLPKHRVIGSGCNLDSARFRYLMAEKLGIHPSSCHGWILGEHGDSSVAVWSGVNVAGVSLQELNPEMGTDNDSENWKEVHKMVVESAYEVIKLKGYTNWAIGLSVADLIESMLKNLSRIHPVSTMVKGMYGIENEVFLSLPCILNARGLTSVINQKLKDDEVAQLKKSADTLWDIQKDLKDL
Gene ID
Swiss Prot
SynonymsLDH-B; LDH-H; LDHBD; TRG-5; HEL-S-281; HB
Calculated MW37kDa
Observed MW36kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samples293T
Cellular locationCytoplasm.
Customer validation

WB(Rattus norvegicus, Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - [KO Validated] LDHB Rabbit pAb (A18096)}

Western blot - [KO Validated] LDHB Rabbit pAb (A18096)

Western blot analysis of lysates from wild type (WT) and LDHB knockout (KO) 293T cells, using [KO Validated] LDHB Rabbit pAb (A18096) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - [KO Validated] LDHB Rabbit pAb (A18096)}

Western blot - [KO Validated] LDHB Rabbit pAb (A18096)

Western blot analysis of lysates from wild type(WT) and LDHB knockout (KO) 293T cells, using [KO Validated] LDHB Rabbit pAb (A18096) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.
ABclonal:Immunofluorescence - [KO Validated] LDHB Rabbit pAb (A18096)}

Immunofluorescence - [KO Validated] LDHB Rabbit pAb (A18096)

Immunofluorescence analysis of Mouse heart tissue using [KO Validated] LDHB Rabbit pAb (A18096) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A18096? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

Secondary Antibodies (26)