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[KO Validated] LITAF Rabbit pAb (A5469)

KO/KDValidated

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - [KO Validated] LITAF Rabbit pAb (A5469)

Western blot analysis of lysates from wild type (WT) and LITAF knockout (KO) HeLa cells, using [KO Validated] LITAF Rabbit pAb (A5469) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - [KO Validated] LITAF Rabbit pAb (A5469)

Western blot analysis of lysates from HeLa cells, using LITAF Rabbit pAb (A5469) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

ABclonal:Immunohistochemistry - [KO Validated] LITAF Rabbit pAb (A5469)

Immunohistochemistry analysis of paraffin-embedded Human prostate using [KO Validated] LITAF Rabbit pAb (A5469) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product name[KO Validated] LITAF Rabbit pAb
Catalog No.A5469
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Lipopolysaccharide is a potent stimulator of monocytes and macrophages, causing secretion of tumor necrosis factor-alpha (TNF-alpha) and other inflammatory mediators. This gene encodes lipopolysaccharide-induced TNF-alpha factor, which is a DNA-binding protein and can mediate the TNF-alpha expression by direct binding to the promoter region of the TNF-alpha gene. The transcription of this gene is induced by tumor suppressor p53 and has been implicated in the p53-induced apoptotic pathway. Mutations in this gene cause Charcot-Marie-Tooth disease type 1C (CMT1C) and may be involved in the carcinogenesis of extramammary Paget's disease (EMPD). Multiple alternatively spliced transcript variants have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-161 of human LITAF (NP_004853.2).
SequenceMSVPGPYQAATGPSSAPSAPPSYEETVAVNSYYPTPPAPMPGPTTGLVTGPDGKGMNPPSYYTQPAPIPNNNPITVQTVYVQHPITFLDRPIQMCCPSCNKMIVSQLSYNAGALTWLSCGSLCLLGCIAGCCFIPFCVDALQDVDHYCPNCRALLGTYKRL
Gene ID
Swiss Prot
SynonymsPIG7; SIMPLE; TP53I7; AF
Calculated MW17kDa
Observed MW23kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunoprecipitation    
Positive samplesHeLa
Cellular locationCytoplasmic side, Lysosome membrane, Peripheral membrane protein

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - [KO Validated] LITAF Rabbit pAb (A5469)}

Western blot - [KO Validated] LITAF Rabbit pAb (A5469)

Western blot analysis of lysates from wild type (WT) and LITAF knockout (KO) HeLa cells, using [KO Validated] LITAF Rabbit pAb (A5469) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - [KO Validated] LITAF Rabbit pAb (A5469)}

Western blot - [KO Validated] LITAF Rabbit pAb (A5469)

Western blot analysis of lysates from HeLa cells, using LITAF Rabbit pAb (A5469) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.
ABclonal:Immunohistochemistry - [KO Validated] LITAF Rabbit pAb (A5469)}

Immunohistochemistry - [KO Validated] LITAF Rabbit pAb (A5469)

Immunohistochemistry analysis of paraffin-embedded Human prostate using [KO Validated] LITAF Rabbit pAb (A5469) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)