Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of lysates from wild type(WT) and LRRC59 knockout (KO) 293T(KO) cells, using [KO Validated] LRRC59 Rabbit pAb (A10022) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded rat cerebellum using LRRC59 Rabbit pAb (A10022) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human brain using LRRC59 Rabbit pAb (A10022) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
| Product name | [KO Validated] LRRC59 Rabbit pAb |
|---|---|
| Catalog No. | A10022 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-244 of human LRRC59 (NP_060979.2). |
|---|---|
| Sequence | MTKAGSKGGNLRDKLDGNELDLSLSDLNEVPVKELAALPKATILDLSCNKLTTLPSDFCGLTHLVKLDLSKNKLQQLPADFGRLVNLQHLDLLNNKLVTLPVSFAQLKNLKWLDLKDNPLDPVLAKVAGDCLDEKQCKQCANKVLQHMKAVQADQERERQRRLEVEREAEKKREAKQRAKEAQERELRKREKAEEKERRRKEYDALKAAKREQEKKPKKEANQAPKSKSGSRPRKPPPRKHTRS |
| Gene ID | |
| Swiss Prot | |
| Synonyms | p34; PRO1855; 59 |
| Calculated MW | 35kDa |
| Observed MW | 35kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunohistochemistry |
| Positive samples | 293T |
| Cellular location | Endoplasmic reticulum membrane, Microsome membrane, Nucleus envelope, Single-pass type II membrane protein |
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* For research use only. Not for therapeutic or diagnostic purposes.
![ABclonal:Western blot - [KO Validated] LRRC59 Rabbit pAb (A10022)](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A10022_P7361_KO-WB_01.jpg?t=1714967453 "ABclonal:Western blot - [KO Validated] LRRC59 Rabbit pAb (A10022)")
![ABclonal:Immunohistochemistry - [KO Validated] LRRC59 Rabbit pAb (A10022)](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A10022_P7361_IHC_01.jpg?t=1714967453 "ABclonal:Immunohistochemistry - [KO Validated] LRRC59 Rabbit pAb (A10022)")
![ABclonal:Immunohistochemistry - [KO Validated] LRRC59 Rabbit pAb (A10022)](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A10022_P7361_IHC_02.jpg?t=1714967453 "ABclonal:Immunohistochemistry - [KO Validated] LRRC59 Rabbit pAb (A10022)")
![ABclonal:Immunohistochemistry - [KO Validated] LRRC59 Rabbit pAb (A10022)](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A10022_P7361_IHC_03.jpg?t=1714967453 "ABclonal:Immunohistochemistry - [KO Validated] LRRC59 Rabbit pAb (A10022)")
