제품 > 항체 > 다중클론항체(pAb)

[KO Validated] Lamin B1 Rabbit pAb (A16909)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using [KO Validated] Lamin B1 Rabbit pAb (A16909) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Western blot analysis of lysates from wild type (WT) and Lamin B1 knockout (KO) HeLa cells, using [KO Validated] Lamin B1 Rabbit pAb (A16909) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product name	[KO Validated] Lamin B1 Rabbit pAb
Catalog No.	A16909
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes one of the two B-type lamin proteins and is a component of the nuclear lamina. A duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 503-586 of human Lamin B1 (NP_005564.1).
Sequence	AGVTASPPTDLIWKNQNSWGTGEDVKVILKNSQGEEVAQRSTVFKTTIPEEEEEEEEAAGVVVEEELFHQQGTPRASNRSCAIM
Gene ID
Swiss Prot
Synonyms	LMN; ADLD; LMN2; LMNB; MCPH26; B1
Calculated MW	66kDa
Observed MW	75kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:5000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	Jurkat, HT-29, HeLa, U-251MG, mouse liver, mouse brain, mouse spleen, rat liver
Cellular location	Lipid-anchor, Nucleoplasmic side, Nucleus inner membrane.
Customer validation	WB(Rattus norvegicus, Homo sapiens, Canis lupus familiaris, Mus musculus) IF(Homo sapiens) Co-IP(Mus musculus) IF(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

연구세포 생물학 & 발달 생물학세포 골격중간섬유

[KO Validated] Lamin B1 Rabbit pAb images

Western blot - [KO Validated] Lamin B1 Rabbit pAb (A16909)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for [KO Validated] Lamin B1 Rabbit pAb (A16909)

Product:

[KO Validated] Lamin B1 Rabbit pAb

Journal:

Phytother Res

Application:

IF:

6.1

Species:

Rattus norvegicus

PMID:

34426999

Title:

Icariin attenuates renal fibrosis in chronic kidney disease by inhibiting interleukin-1β/transforming growth factor-β-mediated activation of renal fibroblasts

References for [KO Validated] Lamin B1 Rabbit pAb (A16909)

Product:

[KO Validated] Lamin B1 Rabbit pAb

Journal:

J Transl Med

Application:

IF:

8.4

Species:

Homo sapiens

PMID:

34627268

Title:

CENPA promotes clear cell renal cell carcinoma progression and metastasis via Wnt/β-catenin signaling pathway

References for [KO Validated] Lamin B1 Rabbit pAb (A16909)

Product:

[KO Validated] Lamin B1 Rabbit pAb

Journal:

Basic & clinical pharmacology & toxicology

Application:

IF:

2.7

Species:

Homo sapiens

PMID:

36585038

Title:

Bortezomib inhibits hepatocellular carcinoma via the Hippo‐YAP signaling pathway

References for [KO Validated] Lamin B1 Rabbit pAb (A16909)

Product:

[KO Validated] Lamin B1 Rabbit pAb

Journal:

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

Application:

IF:

3.9

Species:

Homo sapiens

PMID:

36436616

Title:

PGC-1α/NRF1-dependent cardiac mitochondrial biogenesis: A druggable pathway of calycosin against triptolide cardiotoxicity

References for [KO Validated] Lamin B1 Rabbit pAb (A16909)

Product:

[KO Validated] Lamin B1 Rabbit pAb

Journal:

Antioxidants (Basel, Switzerland)

Application:

IF:

7.7

Species:

Homo sapiens

PMID:

36829835

Title:

RNA G-Quadruplex within the 5-UTR of FEN1 Regulates mRNA Stability under Oxidative Stress - PMC

References for [KO Validated] Lamin B1 Rabbit pAb (A16909)

Product:

[KO Validated] Lamin B1 Rabbit pAb

Journal:

Free radical biology & medicine

Application:

IF:

7.1

Species:

Rattus norvegicus

PMID:

36764626

Title:

BDNF mimetic 7, 8-dihydroxyflavone rescues rotenone-induced cytotoxicity in cardiomyocytes by ameliorating mitochondrial dysfunction

References for [KO Validated] Lamin B1 Rabbit pAb (A16909)

Product:

[KO Validated] Lamin B1 Rabbit pAb

Journal:

Redox biology

Application:

IF:

10.7

Species:

Homo sapiens

PMID:

36989576

Title:

Glutaredoxin 1 protects lens epithelial cells from epithelial-mesenchymal transition by preventing casein kinase 1α S-glutathionylation during posterior capsular opacification - PMC

References for [KO Validated] Lamin B1 Rabbit pAb (A16909)

Product:

[KO Validated] Lamin B1 Rabbit pAb

Journal:

Cell death discovery

Application:

IF:

7.1

Species:

Canis lupus familiaris

PMID:

37863897

Title:

YAP nuclear translocation induced by HIF-1 prevents DNA damage under hypoxic conditions - PMC

References for [KO Validated] Lamin B1 Rabbit pAb (A16909)

Product:

[KO Validated] Lamin B1 Rabbit pAb

Journal:

bioRxiv : the preprint server for biology

Application:

IF:

Species:

Homo sapiens

PMID:

37503005

Title:

Eye lens organoids going simple: characterization of a new 3-dimensional organoid model for lens development and pathology

References for [KO Validated] Lamin B1 Rabbit pAb (A16909)

Product:

[KO Validated] Lamin B1 Rabbit pAb

Journal:

European journal of cell biology

Application:

WB, IF

IF:

4.5

Species:

Homo sapiens

PMID:

37302175

Title:

Depletion of intracellular Ca2+ induces FOXM1 SUMOylation and accumulation on the inner nuclear membrane and accelerates G2/M cell cycle transition

References for [KO Validated] Lamin B1 Rabbit pAb (A16909)

Product:

[KO Validated] Lamin B1 Rabbit pAb

Journal:

iScience

Application:

IF:

5.8

Species:

Homo sapiens

PMID:

36594018

Title:

Genetic ablation of diabetes-associated gene Ccdc92 reduces obesity and insulin resistance in mice - PMC

References for [KO Validated] Lamin B1 Rabbit pAb (A16909)

Product:

[KO Validated] Lamin B1 Rabbit pAb

Journal:

Experimental cell research

Application:

IF:

3.3

Species:

Homo sapiens

PMID:

36341908

Title:

Inhibition of 14-3-3 by K50 acetylation activates YAP1 to promote cholangiocarcinoma growth

References for [KO Validated] Lamin B1 Rabbit pAb (A16909)

Product:

[KO Validated] Lamin B1 Rabbit pAb

Journal:

Oncogene

Application:

IF:

6.9

Species:

Homo sapiens

PMID:

38216672

Title:

TKT-PARP1 axis induces radioresistance by promoting DNA double-strand break repair in hepatocellular carcinoma - PMC

References for [KO Validated] Lamin B1 Rabbit pAb (A16909)

Product:

[KO Validated] Lamin B1 Rabbit pAb

Journal:

The EMBO Journal

Application:

WB, Co-IP

IF:

9.4

Species:

Mus musculus

PMID:

32301534

Title:

SIRT5 impairs aggregation and activation of the signaling adaptor MAVS through catalyzing lysine desuccinylation

References for [KO Validated] Lamin B1 Rabbit pAb (A16909)

Product:

[KO Validated] Lamin B1 Rabbit pAb

Journal:

Cell death & disease

Application:

IF:

8.1

Species:

Homo sapiens

PMID:

35902564

Title:

The interaction between STING and NCOA4 exacerbates lethal sepsis by orchestrating ferroptosis and inflammatory responses in macrophages - PMC

References for [KO Validated] Lamin B1 Rabbit pAb (A16909)

Product:

[KO Validated] Lamin B1 Rabbit pAb

Journal:

Aging and disease

Application:

IF:

Species:

Mus musculus

PMID:

38447216

Title:

An Antibody-directed and Immune Response Modifier-augmented Photothermal Therapy Strategy Relieves Aging via Rapid Immune Clearance of Senescent Cells - PMC

References for [KO Validated] Lamin B1 Rabbit pAb (A16909)

Product:

[KO Validated] Lamin B1 Rabbit pAb

Journal:

Journal of extracellular vesicles

Application:

IF:

Species:

Homo sapiens

PMID:

39235072

Title:

The 8-oxoguanine DNA glycosylase-synaptotagmin 7 pathway increases extracellular vesicle release and promotes tumour metastasis during oxidative stress - PMC

References for [KO Validated] Lamin B1 Rabbit pAb (A16909)

Product:

[KO Validated] Lamin B1 Rabbit pAb

Journal:

Cell death & disease

Application:

IF:

8.1

Species:

Homo sapiens

PMID:

39198402