Western blot - [KO Validated] MAP2K4 Rabbit pAb (A14781)
Western blot analysis of various lysates using [KO Validated] MAP2K4 Rabbit pAb (A14781) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Western blot - [KO Validated] MAP2K4 Rabbit pAb (A14781)
Western blot analysis of lysates from wild type (WT) and MAP2K4 knockout (KO) HeLa cells, using [KO Validated] MAP2K4 Rabbit pAb (A14781) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
Western blot - [KO Validated] MAP2K4 Rabbit pAb (A14781)
Western blot analysis of lysates from A-549 cells, using MAP2K4 Rabbit pAb (A14781) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Western blot - [KO Validated] MAP2K4 Rabbit pAb (A14781)
Western blot analysis of various lysates, using MAP2K4 Rabbit pAb (A14781) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Western blot - [KO Validated] MAP2K4 Rabbit pAb (A14781)
Western blot analysis of lysates from HeLa cells, using MAP2K4 Rabbit pAb (A14781) at 1:700 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
Immunohistochemistry - [KO Validated] MAP2K4 Rabbit pAb (A14781)
Immunohistochemistry analysis of paraffin-embedded Mouse liver using MAP2K4 Rabbit pAb (A14781) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry - [KO Validated] MAP2K4 Rabbit pAb (A14781)
Immunohistochemistry analysis of paraffin-embedded Rat ovary using MAP2K4 Rabbit pAb (A14781) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry - [KO Validated] MAP2K4 Rabbit pAb (A14781)
Immunohistochemistry analysis of paraffin-embedded Mouse kidney using MAP2K4 Rabbit pAb (A14781) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry - [KO Validated] MAP2K4 Rabbit pAb (A14781)
Immunohistochemistry analysis of paraffin-embedded Human colon cancer using MAP2K4 Rabbit pAb (A14781) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry - [KO Validated] MAP2K4 Rabbit pAb (A14781)
Immunohistochemistry analysis of paraffin-embedded Rat lung using MAP2K4 Rabbit pAb (A14781) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.