Western blot - Phospho-MAP2K4-S257 Rabbit mAb (AP1491)
Western blot analysis of lysates from C6 cells using Phospho-MAP2K4-S257 Rabbit mAb (AP1491) at 1:2600 dilution incubated overnight at 4℃. C6 cells were treated by UV (90mJ/cm2) at room temperature and recovered for 2 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
Immunohistochemistry - Phospho-MAP2K4-S257 Rabbit mAb (AP1491)
Immunohistochemistry analysis of paraffin-embedded Human colon tissue, Untreated (left) and lambda phosphatase-treated (right), using Phospho-MAP2K4-S257 Rabbit mAb (AP1491) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - Phospho-MAP2K4-S257 Rabbit mAb (AP1491)
Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue, Untreated (left) and lambda phosphatase-treated (right), using Phospho-MAP2K4-S257 Rabbit mAb (AP1491) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - Phospho-MAP2K4-S257 Rabbit mAb (AP1491)
Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue, Untreated (left) and lambda phosphatase-treated (right), using Phospho-MAP2K4-S257 Rabbit mAb (AP1491) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - Phospho-MAP2K4-S257 Rabbit mAb (AP1491)
Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Phospho-MAP2K4-S257 Rabbit mAb (AP1491) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - Phospho-MAP2K4-S257 Rabbit mAb (AP1491)
Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using Phospho-MAP2K4-S257 Rabbit mAb (AP1491) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - Phospho-MAP2K4-S257 Rabbit mAb (AP1491)
Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using Phospho-MAP2K4-S257 Rabbit mAb (AP1491) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunofluorescence - Phospho-MAP2K4-S257 Rabbit mAb (AP1491)
Confocal imaging of HeLa cells (treated with UV) and HeLa cells (untreated) using Phospho-MAP2K4-S257 Rabbit mAb (AP1491, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
Immunoprecipitation - Phospho-MAP2K4-S257 Rabbit mAb (AP1491)
Immunoprecipitation of Phospho-MAP2K4-S257 from 200 µg extracts of HeLa cells treated by UV (100 mJ/cm2, 4 hours) was performed using 0.5 µg of Phospho-MAP2K4-S257 Rabbit mAb (AP1491). Rabbit IgG isotype control (AC042) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using Phospho-MAP2K4-S257 Rabbit mAb (AP1491) at a dilution of 1:1000.