Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of various lysates using MPG Rabbit pAb (A18070) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 40s.
Western blot analysis of lysates from wild type (WT) and MPG knockout (KO) HeLa cells, using [KO Validated] MPG Rabbit pAb (A18070) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
| Product name | [KO Validated] MPG Rabbit pAb |
|---|---|
| Catalog No. | A18070 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-293 of human MPG (NP_001015052.1). |
|---|---|
| Sequence | MPARSGAQFCRRMGQKKQRPARAGQPHSSSDAAQAPAEQPHSSSDAAQAPCPRERCLGPPTTPGPYRSIYFSSPKGHLTRLGLEFFDQPAVPLARAFLGQVLVRRLPNGTELRGRIVETEAYLGPEDEAAHSRGGRQTPRNRGMFMKPGTLYVYIIYGMYFCMNISSQGDGACVLLRALEPLEGLETMRQLRSTLRKGTASRVLKDRELCSGPSKLCQALAINKSFDQRDLAQDEAVWLERGPLEPSEPAVVAAARVGVGHAGEWARKPLRFYVRGSPWVSVVDRVAEQDTQA |
| Gene ID | |
| Swiss Prot | |
| Synonyms | AAG; MDG; ADPG; APNG; Mid1; anpg; PIG11; PIG16; CRA36.1; PG |
| Calculated MW | 33kDa |
| Observed MW | 37kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Key application | Western blotting |
| Positive samples | HeLa |
| Cellular location | Cytoplasm, Mitochondrion matrix, Nucleus, mitochondrion nucleoid. |
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![ABclonal:Western blot - [KO Validated] MPG Rabbit pAb (A18070)](https://img.abclonal.com/abclonal-manage/Catalog/A18070/A18070_1.jpg?t=1725334856 "ABclonal:Western blot - [KO Validated] MPG Rabbit pAb (A18070)")
![ABclonal:Western blot - [KO Validated] MPG Rabbit pAb (A18070)](https://img.abclonal.com/abclonal-manage/Catalog/A18070/A18070_2.jpg?t=1725334856 "ABclonal:Western blot - [KO Validated] MPG Rabbit pAb (A18070)")
