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[KO Validated] MTA2 Rabbit pAb (A2243)

KO/KDValidated

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] MTA2 Rabbit pAb (A2243)

Western blot analysis of lysates from wild type (WT) and MTA2 knockout (KO) 293T cells, using [KO Validated] MTA2 Rabbit pAb (A2243) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Chromatin Immunoprecipitation - [KO Validated] MTA2 Rabbit pAb (A2243)

Chromatin immunoprecipitation analysis of extracts of HeLa cells, using MTA2 antibody (A2243) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Overview

Product name[KO Validated] MTA2 Rabbit pAb
Catalog No.A2243
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein that has been identified as a component of NuRD, a nucleosome remodeling deacetylase complex identified in the nucleus of human cells. It shows a very broad expression pattern and is strongly expressed in many tissues. It may represent one member of a small gene family that encode different but related proteins involved either directly or indirectly in transcriptional regulation. Their indirect effects on transcriptional regulation may include chromatin remodeling. It is closely related to another member of this family, a protein that has been correlated with the metastatic potential of certain carcinomas. These two proteins are so closely related that they share the same types of domains. These domains include two DNA binding domains, a dimerization domain, and a domain commonly found in proteins that methylate DNA. One of the proteins known to be a target protein for this gene product is p53. Deacetylation of p53 is correlated with a loss of growth inhibition in transformed cells supporting a connection between these gene family members and metastasis.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 60-180 of human MTA2 (NP_004730.2).
SequenceDSNAREFEEESKQPGVSEQQRHQLKHRELFLSRQFESLPATHIRGKCSVTLLNETDILSQYLEKEDCFFYSLVFDPVQKTLLADQGEIRVGCKYQAEIPDRLVEGESDNRNQQKMEMKVWD
Gene ID
Swiss Prot
SynonymsPID; MTA1L1; A2
Calculated MW75kDa
Observed MW75kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
  • ChIP 5μg antibody for 10μg-15μg of Chromatin
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T
Cellular locationNucleus.
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - [KO Validated] MTA2 Rabbit pAb (A2243)}

Western blot - [KO Validated] MTA2 Rabbit pAb (A2243)

Western blot analysis of lysates from wild type (WT) and MTA2 knockout (KO) 293T cells, using [KO Validated] MTA2 Rabbit pAb (A2243) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Chromatin Immunoprecipitation - [KO Validated] MTA2 Rabbit pAb (A2243)}

Chromatin Immunoprecipitation - [KO Validated] MTA2 Rabbit pAb (A2243)

Chromatin immunoprecipitation analysis of extracts of HeLa cells, using MTA2 antibody (A2243) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A2243? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

Secondary Antibodies (26)