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[KO Validated] NHEJ1 Rabbit pAb (A19957)

KO/KDValidated

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - [KO Validated] NHEJ1 Rabbit pAb (A19957)

Western blot analysis of lysates from wild type (WT) and NHEJ1 knockout (KO) HeLa cells, using [KO Validated] NHEJ1 Rabbit pAb (A19957) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

Overview

Product name[KO Validated] NHEJ1 Rabbit pAb
Catalog No.A19957
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-299 of human NHEJ1 (NP_079058.1).
SequenceMEELEQGLLMQPWAWLQLAENSLLAKVFITKQGYALLVSDLQQVWHEQVDTSVVSQRAKELNKRLTAPPAAFLCHLDNLLRPLLKDAAHPSEATFSCDCVADALILRVRSELSGLPFYWNFHCMLASPSLVSQHLIRPLMGMSLALQCQVRELATLLHMKDLEIQDYQESGATLIRDRLKTEPFEENSFLEQFMIEKLPEACSIGDGKPFVMNLQDLYMAVTTQEVQVGQKHQGAGDPHTSNSASLQGIDSQCVNQPEQLVSSAPTLSAPEKESTGTSGPLQRPQLSKVKRKKPRGLFS
Gene ID
Swiss Prot
SynonymsXLF; J1
Calculated MW33kDa
Observed MW39kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:200 - 1:2000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa
Cellular locationNucleus.
Customer validation

WB(Homo sapiens)

ABclonal:Western blot - [KO Validated] NHEJ1 Rabbit pAb (A19957)}

Western blot - [KO Validated] NHEJ1 Rabbit pAb (A19957)

Western blot analysis of lysates from wild type (WT) and NHEJ1 knockout (KO) HeLa cells, using [KO Validated] NHEJ1 Rabbit pAb (A19957) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (1)

Secondary Antibodies (25)