Western blot - [KO Validated] NRBF2 Rabbit pAb (A6462)
Western blot analysis of various lysates using NRBF2 Rabbit pAb (A6462) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
Western blot - [KO Validated] NRBF2 Rabbit pAb (A6462)
Western blot analysis of lysates from HepG2 cells, using [KO Validated] NRBF2 Rabbit pAb (A6462) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
Western blot - [KO Validated] NRBF2 Rabbit pAb (A6462)
Western blot analysis of lysates from wild type (WT) and NRBF2 knockout (KO) 293T cells, using [KO Validated] NRBF2 Rabbit pAb (A6462) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Immunohistochemistry - [KO Validated] NRBF2 Rabbit pAb (A6462)
Immunohistochemistry analysis of paraffin-embedded Human esophageal using [KO Validated] NRBF2 Rabbit pAb (A6462) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Immunofluorescence - [KO Validated] NRBF2 Rabbit pAb (A6462)
Immunofluorescence analysis of C6 cells using [KO Validated] NRBF2 Rabbit pAb (A6462) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence - [KO Validated] NRBF2 Rabbit pAb (A6462)
Immunofluorescence analysis of L929 cells using [KO Validated] NRBF2 Rabbit pAb (A6462) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence - [KO Validated] NRBF2 Rabbit pAb (A6462)
Immunofluorescence analysis of U-2 OS cells using [KO Validated] NRBF2 Rabbit pAb (A6462) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence - [KO Validated] NRBF2 Rabbit pAb (A6462)
Immunofluorescence analysis of C6 cells using NRBF2 Rabbit pAb (A6462) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence - [KO Validated] NRBF2 Rabbit pAb (A6462)
Immunofluorescence analysis of HeLa cells using NRBF2 Rabbit pAb (A6462) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence - [KO Validated] NRBF2 Rabbit pAb (A6462)
Immunofluorescence analysis of NIH-3T3 cells using NRBF2 Rabbit pAb (A6462) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.