Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of various lysates using [KO Validated] NUCB1 Rabbit pAb (A3994) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Western blot analysis of lysates from wild type (WT) and NUCB1 knockout (KO) 293T cells, using [KO Validated] NUCB1 Rabbit pAb (A3994) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
Immunofluorescence analysis of L929 cells using [KO Validated] NUCB1 Rabbit pAb (A3994) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
| Product name | [KO Validated] NUCB1 Rabbit pAb |
|---|---|
| Catalog No. | A3994 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 302-461 of human NUCB1 (NP_006175.2). |
|---|---|
| Sequence | KNVDTNQDRLVTLEEFLASTQRKEFGDTGEGWETVEMHPAYTEEELRRFEEELAAREAELNAKAQRLSQETEALGRSQGRLEAQKRELQQAVLHMEQRKQQQQQQQGHKAPAAHPEGQLKFHPDTDDVPVPAPAGDQKEVDTSEKKLLERLPEVEVPQHL |
| Gene ID | |
| Swiss Prot | |
| Synonyms | NUC; CALNUC; B1 |
| Calculated MW | 54kDa |
| Observed MW | 60kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunofluorescence |
| Positive samples | A-549, HepG2, 293T, BxPC-3 |
| Cellular location | Cytoplasm, Golgi apparatus, Lumenal side, Peripheral membrane protein, cis-Golgi network membrane |
To download a Certificate of Compliance, please enter your Lot number below:
Lot number
* For research use only. Not for therapeutic or diagnostic purposes.
![ABclonal:Western blot - [KO Validated] NUCB1 Rabbit pAb (A3994)](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A3994_P11986_WB_01.jpg?t=1709198900 "ABclonal:Western blot - [KO Validated] NUCB1 Rabbit pAb (A3994)")
![ABclonal:Western blot - [KO Validated] NUCB1 Rabbit pAb (A3994)](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A3994_P11986_KO-WB_01.jpg?t=1709198900 "ABclonal:Western blot - [KO Validated] NUCB1 Rabbit pAb (A3994)")
![ABclonal:Immunofluorescence - [KO Validated] NUCB1 Rabbit pAb (A3994)](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A3994_P11986_IF_01.jpg?t=1709198900 "ABclonal:Immunofluorescence - [KO Validated] NUCB1 Rabbit pAb (A3994)")
![ABclonal:Immunofluorescence - [KO Validated] NUCB1 Rabbit pAb (A3994)](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A3994_P11986_IF_02.jpg?t=1709198900 "ABclonal:Immunofluorescence - [KO Validated] NUCB1 Rabbit pAb (A3994)")
