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[KO Validated] PCCB Rabbit mAb (A24917)

KO/KDValidated

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] PCCB Rabbit mAb (A24917)

Western blot analysis of lysates from wild type (WT) and PCCB knockout (KO) 293T cells, using [KO Validated] PCCB (A24917) at 1:6000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - [KO Validated] PCCB Rabbit mAb (A24917)

Immunohistochemistry analysis of PCCB in paraffin-embedded human liver tissue using [KO Validated] PCCB Rabbit mAb (A24917) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KO Validated] PCCB Rabbit mAb (A24917)

Immunohistochemistry analysis of PCCB in paraffin-embedded mouse kidney tissue using [KO Validated] PCCB Rabbit mAb (A24917) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KO Validated] PCCB Rabbit mAb (A24917)

Immunohistochemistry analysis of PCCB in paraffin-embedded rat kidney tissue using [KO Validated] PCCB Rabbit mAb (A24917) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - [KO Validated] PCCB Rabbit mAb (A24917)

Confocal imaging of HEL cells using [KO Validated] PCCB Rabbit mAb (A24917, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

Overview

Product name[KO Validated] PCCB Rabbit mAb
Catalog No.A24917
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC64902
The protein encoded by this gene is a subunit of the propionyl-CoA carboxylase (PCC) enzyme, which is involved in the catabolism of propionyl-CoA. PCC is a mitochondrial enzyme that probably acts as a dodecamer of six alpha subunits and six beta subunits. This gene encodes the beta subunit of PCC. Defects in this gene are a cause of propionic acidemia type II (PA-2). Multiple transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 260-539 of Human PCCB(NP_000523.2).
SequenceAFENDVDALCNLRDFFNYLPLSSQDPAPVRECHDPSDRLVPELDTIVPLESTKAYNMVDIIHSVVDEREFFEIMPNYAKNIIVGFARMNGRTVGIVGNQPKVASGCLDINSSVKGARFVRFCDAFNIPLITFVDVPGFLPGTAQEYGGIIRHGAKLLYAFAEATVPKVTVITRKAYGGAYDVMSSKHLCGDTNYAWPTAEIAVMGAKGAVEIIFKGHENVEAAQAEYIEKFANPFPAAVRGFVDDIIQPSSTRARICCDLDVLASKKVQRPWRKHANIPL
Gene ID
Swiss Prot
SynonymsPCCB; CB
Calculated MW58kDa
Observed MW58kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:7000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T
Cellular locationMitochondrion matrix

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - [KO Validated] PCCB Rabbit mAb (A24917)}

Western blot - [KO Validated] PCCB Rabbit mAb (A24917)

Western blot analysis of lysates from wild type (WT) and PCCB knockout (KO) 293T cells, using [KO Validated] PCCB (A24917) at 1:6000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - [KO Validated] PCCB Rabbit mAb (A24917)}

Immunohistochemistry - [KO Validated] PCCB Rabbit mAb (A24917)

Immunohistochemistry analysis of PCCB in paraffin-embedded human liver tissue using [KO Validated] PCCB Rabbit mAb (A24917) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KO Validated] PCCB Rabbit mAb (A24917)}

Immunohistochemistry - [KO Validated] PCCB Rabbit mAb (A24917)

Immunohistochemistry analysis of PCCB in paraffin-embedded mouse kidney tissue using [KO Validated] PCCB Rabbit mAb (A24917) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KO Validated] PCCB Rabbit mAb (A24917)}

Immunohistochemistry - [KO Validated] PCCB Rabbit mAb (A24917)

Immunohistochemistry analysis of PCCB in paraffin-embedded rat kidney tissue using [KO Validated] PCCB Rabbit mAb (A24917) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - [KO Validated] PCCB Rabbit mAb (A24917)}

Immunofluorescence - [KO Validated] PCCB Rabbit mAb (A24917)

Confocal imaging of HEL cells using [KO Validated] PCCB Rabbit mAb (A24917, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)