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[KO Validated] PRKAR1A Rabbit pAb (A0906)

KO/KDValidated

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] PRKAR1A Rabbit pAb (A0906)

Western blot analysis of various lysates using [KO Validated] PRKAR1A Rabbit pAb (A0906) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Western blot - [KO Validated] PRKAR1A Rabbit pAb (A0906)

Western blot analysis of lysates from wild type (WT) and PRKAR1A knockout (KO) 293T cells, using [KO Validated] PRKAR1A Rabbit pAb (A0906) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Western blot - [KO Validated] PRKAR1A Rabbit pAb (A0906)

Western blot analysis of lysates from wild type (WT) and PRKAR1A knockout (KO) 293T cells, using [KO Validated] PRKAR1A Rabbit pAb (A0906) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Western blot - [KO Validated] PRKAR1A Rabbit pAb (A0906)

Western blot analysis of lysates from Mouse testis using PRKAR1A Rabbit pAb (A0906) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - [KO Validated] PRKAR1A Rabbit pAb (A0906)

Immunofluorescence analysis of NIH/3T3 cells using PRKAR1A Rabbit pAb (A0906) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name[KO Validated] PRKAR1A Rabbit pAb
Catalog No.A0906
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. This gene encodes one of the regulatory subunits. This protein was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Mutations in this gene cause Carney complex (CNC). This gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2. A nonconventional nuclear localization sequence (NLS) has been found for this protein which suggests a role in DNA replication via the protein serving as a nuclear transport protein for the second subunit of the Replication Factor C (RFC40). Several alternatively spliced transcript variants encoding two different isoforms have been observed.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human PRKAR1A (NP_002725.1).
SequenceMESGSTAASEEARSLRECELYVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEKEEAKQIQNLQKAGTRTDSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFSVSFIAGETVIQQGDEGDNFYVIDQGETDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFL
Gene ID
Swiss Prot
SynonymsCAR; CNC; CNC1; PKR1; TSE1; ADOHR; PPNAD1; PRKAR1; ACRDYS1; 1A
Calculated MW43kDa
Observed MW48kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samples293T, HeLa, PC-3, U-251MG
Cellular locationCell membrane.
Customer validation

WB(Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - [KO Validated] PRKAR1A Rabbit pAb (A0906)}

Western blot - [KO Validated] PRKAR1A Rabbit pAb (A0906)

Western blot analysis of various lysates using [KO Validated] PRKAR1A Rabbit pAb (A0906) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Western blot - [KO Validated] PRKAR1A Rabbit pAb (A0906)}

Western blot - [KO Validated] PRKAR1A Rabbit pAb (A0906)

Western blot analysis of lysates from wild type (WT) and PRKAR1A knockout (KO) 293T cells, using [KO Validated] PRKAR1A Rabbit pAb (A0906) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Western blot - [KO Validated] PRKAR1A Rabbit pAb (A0906)}

Western blot - [KO Validated] PRKAR1A Rabbit pAb (A0906)

Western blot analysis of lysates from wild type (WT) and PRKAR1A knockout (KO) 293T cells, using [KO Validated] PRKAR1A Rabbit pAb (A0906) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Western blot - [KO Validated] PRKAR1A Rabbit pAb (A0906)}

Western blot - [KO Validated] PRKAR1A Rabbit pAb (A0906)

Western blot analysis of lysates from Mouse testis using PRKAR1A Rabbit pAb (A0906) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunofluorescence - [KO Validated] PRKAR1A Rabbit pAb (A0906)}

Immunofluorescence - [KO Validated] PRKAR1A Rabbit pAb (A0906)

Immunofluorescence analysis of NIH/3T3 cells using PRKAR1A Rabbit pAb (A0906) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A0906? Please let us know so that we can cite the reference in this datasheet.

Secondary Antibodies (26)