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[KO Validated] RhoC Rabbit pAb (A1062)

KO/KDValidated

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Rat

ABclonal:Western blot - [KO Validated] RhoC Rabbit pAb (A1062)

Western blot analysis of extracts from normal (control) and RhoC knockout (KO) 293T cells, using RhoC antibody (A1062) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 300s.

ABclonal:Western blot - [KO Validated] RhoC Rabbit pAb (A1062)

Western blot analysis of extracts of Rat heart cells, using RhoC antibody (A1062) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product name[KO Validated] RhoC Rabbit pAb
Catalog No.A1062
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human RhoC (NP_786886.1).
SequenceMAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYIADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRQDEHTRRELAKMKQEPVRSEEGRDMANRISAFGYLECSAKTKEGVREVFEMATRAGLQVRKNKRRRGCPIL
Gene ID
Swiss Prot
SynonymsH9; ARH9; ARHC; RHOH9
Calculated MW22kDa
Observed MW22kDa
ReactivityHuman, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T, Rat heart
Cellular locationCell membrane, Cleavage furrow, Cytoplasmic side, Lipid-anchor
Customer validation

WB(Homo sapiens, Mus musculus)

IF(Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - [KO Validated] RhoC Rabbit pAb (A1062)}

Western blot - [KO Validated] RhoC Rabbit pAb (A1062)

Western blot analysis of extracts from normal (control) and RhoC knockout (KO) 293T cells, using RhoC antibody (A1062) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 300s.
ABclonal:Western blot - [KO Validated] RhoC Rabbit pAb (A1062)}

Western blot - [KO Validated] RhoC Rabbit pAb (A1062)

Western blot analysis of extracts of Rat heart cells, using RhoC antibody (A1062) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A1062? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

ELISA 키트 (1)

Secondary Antibodies (26)