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[KO Validated] SELENBP1 Rabbit pAb (A1222)

KO/KDValidated

Review (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - [KO Validated] SELENBP1 Rabbit pAb (A1222)

Western blot analysis of extracts of various cell lines, using SELENBP1 antibody (A1222) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).

ABclonal:Western blot - [KO Validated] SELENBP1 Rabbit pAb (A1222)

Western blot analysis of extracts from normal (control) and SELENBP1 knockout (KO) HeLa cells, using SELENBP1 antibody (A1222) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunofluorescence - [KO Validated] SELENBP1 Rabbit pAb (A1222)

Immunofluorescence analysis of HeLa cells using SELENBP1 antibody (A1222). Blue: DAPI for nuclear staining.

Review
ABclonal: review for [KO Validated] SELENBP1 Rabbit pAb(A1222)

Overview

Product name[KO Validated] SELENBP1 Rabbit pAb
Catalog No.A1222
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the selenium-binding protein family. Selenium is an essential nutrient that exhibits potent anticarcinogenic properties, and deficiency of selenium may cause certain neurologic diseases. The effects of selenium in preventing cancer and neurologic diseases may be mediated by selenium-binding proteins, and decreased expression of this gene may be associated with several types of cancer. The encoded protein may play a selenium-dependent role in ubiquitination/deubiquitination-mediated protein degradation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 243-472 of human SELENBP1 (NP_003935.2).
SequenceSLKDGLIPLEIRFLHNPDAAQGFVGCALSSTIQRFYKNEGGTWSVEKVIQVPPKKVKGWLLPEMPGLITDILLSLDDRFLYFSNWLHGDLRQYDISDPQRPRLTGQLFLGGSIVKGGPVQVLEDEELKSQPEPLVVKGKRVAGGPQMIQLSLDGKRLYITTSLYSAWDKQFYPDLIREGSVMLQVDVDTVKGGLKLNPNFLVDFGKEPLGPALAHELRYPGGDCSSDIWI
Gene ID
Swiss Prot
SynonymsMTO; LPSB; SP56; hSBP; EHMTO; SBP56; HEL-S-134P
Calculated MW52kDa
Observed MW55kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMCF7, Mouse liver, Mouse kideny
Cellular locationCytoplasm, Membrane, Nucleus, Peripheral membrane protein, cytosol
Customer validation

(Human cells)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - [KO Validated] SELENBP1 Rabbit pAb (A1222)}

    Western blot - [KO Validated] SELENBP1 Rabbit pAb (A1222)

    Western blot analysis of extracts of various cell lines, using SELENBP1 antibody (A1222) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    ABclonal:Western blot - [KO Validated] SELENBP1 Rabbit pAb (A1222)}

    Western blot - [KO Validated] SELENBP1 Rabbit pAb (A1222)

    Western blot analysis of extracts from normal (control) and SELENBP1 knockout (KO) HeLa cells, using SELENBP1 antibody (A1222) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 5s.
    ABclonal:Immunofluorescence - [KO Validated] SELENBP1 Rabbit pAb (A1222)}

    Immunofluorescence - [KO Validated] SELENBP1 Rabbit pAb (A1222)

    Immunofluorescence analysis of HeLa cells using SELENBP1 antibody (A1222). Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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