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[KO Validated] SEPHS1 Rabbit pAb (A13403)

KO/KDValidated

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] SEPHS1 Rabbit pAb (A13403)

Western blot analysis of various lysates using SEPHS1 Rabbit pAb (A13403) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - [KO Validated] SEPHS1 Rabbit pAb (A13403)

Western blot analysis of lysates from wild type(WT) and SEPHS1 knockout (KO) 293T(KO) cells, using [KO Validated] SEPHS1 Rabbit pAb (A13403) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - [KO Validated] SEPHS1 Rabbit pAb (A13403)

Immunohistochemistry analysis of paraffin-embedded Human breast using SEPHS1 Rabbit pAb (A13403) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - [KO Validated] SEPHS1 Rabbit pAb (A13403)

Immunohistochemistry analysis of paraffin-embedded Human leiomyoma of uterus using SEPHS1 Rabbit pAb (A13403) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - [KO Validated] SEPHS1 Rabbit pAb (A13403)

Immunofluorescence analysis of HeLa cells using SEPHS1 Rabbit pAb (A13403). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - [KO Validated] SEPHS1 Rabbit pAb (A13403)

Immunofluorescence analysis of MCF7 cells using [KO Validated] SEPHS1 Rabbit pAb (A13403). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name[KO Validated] SEPHS1 Rabbit pAb
Catalog No.A13403
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes an enzyme that synthesizes selenophosphate from selenide and ATP. Selenophosphate is the selenium donor used to synthesize selenocysteine, which is co-translationally incorporated into selenoproteins at in-frame UGA codons.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-392 of human SEPHS1 (NP_036379.2).
SequenceMSTRESFNPESYELDKSFRLTRFTELKGTGCKVPQDVLQKLLESLQENHFQEDEQFLGAVMPRLGIGMDTCVIPLRHGGLSLVQTTDYIYPIVDDPYMMGRIACANVLSDLYAMGVTECDNMLMLLGVSNKMTDRERDKVMPLIIQGFKDAAEEAGTSVTGGQTVLNPWIVLGGVATTVCQPNEFIMPDNAVPGDVLVLTKPLGTQVAVAVHQWLDIPEKWNKIKLVVTQEDVELAYQEAMMNMARLNRTAAGLMHTFNAHAATDITGFGILGHAQNLAKQQRNEVSFVIHNLPVLAKMAAVSKACGNMFGLMHGTCPETSGGLLICLPREQAARFCAEIKSPKYGEGHQAWIIGIVEKGNRTARIIDKPRIIEVAPQVATQNVNPTPGATS
Gene ID
Swiss Prot
SynonymsSPS; SELD; SPS1; S1
Calculated MW43kDa
Observed MW40kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T, BT-474, SW620, HepG2, Rat kidney
Cellular locationCell membrane, Cytoplasm, Nucleus membrane.

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - [KO Validated] SEPHS1 Rabbit pAb (A13403)}

    Western blot - [KO Validated] SEPHS1 Rabbit pAb (A13403)

    Western blot analysis of various lysates using SEPHS1 Rabbit pAb (A13403) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Western blot - [KO Validated] SEPHS1 Rabbit pAb (A13403)}

    Western blot - [KO Validated] SEPHS1 Rabbit pAb (A13403)

    Western blot analysis of lysates from wild type(WT) and SEPHS1 knockout (KO) 293T(KO) cells, using [KO Validated] SEPHS1 Rabbit pAb (A13403) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunohistochemistry - [KO Validated] SEPHS1 Rabbit pAb (A13403)}

    Immunohistochemistry - [KO Validated] SEPHS1 Rabbit pAb (A13403)

    Immunohistochemistry analysis of paraffin-embedded Human breast using SEPHS1 Rabbit pAb (A13403) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunohistochemistry - [KO Validated] SEPHS1 Rabbit pAb (A13403)}

    Immunohistochemistry - [KO Validated] SEPHS1 Rabbit pAb (A13403)

    Immunohistochemistry analysis of paraffin-embedded Human leiomyoma of uterus using SEPHS1 Rabbit pAb (A13403) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunofluorescence - [KO Validated] SEPHS1 Rabbit pAb (A13403)}

    Immunofluorescence - [KO Validated] SEPHS1 Rabbit pAb (A13403)

    Immunofluorescence analysis of HeLa cells using SEPHS1 Rabbit pAb (A13403). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - [KO Validated] SEPHS1 Rabbit pAb (A13403)}

    Immunofluorescence - [KO Validated] SEPHS1 Rabbit pAb (A13403)

    Immunofluorescence analysis of MCF7 cells using [KO Validated] SEPHS1 Rabbit pAb (A13403). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.