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[KO Validated] SERPINB5 Rabbit pAb (A1179)

KO/KDValidated

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - [KO Validated] SERPINB5 Rabbit pAb (A1179)

Western blot analysis of various lysates using SERPINB5 Rabbit pAb (A1179) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Western blot - [KO Validated] SERPINB5 Rabbit pAb (A1179)

Western blot analysis of lysates from wild type (WT) and SERPINB5 knockout (KO) HeLa cells, using [KO Validated] SERPINB5 Rabbit pAb (A1179) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Western blot - [KO Validated] SERPINB5 Rabbit pAb (A1179)

Western blot analysis of lysates from wild type(WT) and SERPINB5 knockout (KO) HeLa(KO) cells, using [KO Validated] SERPINB5 Rabbit pAb (A1179) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunohistochemistry - [KO Validated] SERPINB5 Rabbit pAb (A1179)

Immunohistochemistry analysis of paraffin-embedded Human stomach using SERPINB5 Rabbit pAb (A1179) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - [KO Validated] SERPINB5 Rabbit pAb (A1179)

Immunohistochemistry analysis of paraffin-embedded Human rectum using SERPINB5 Rabbit pAb (A1179) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Overview

Product name[KO Validated] SERPINB5 Rabbit pAb
Catalog No.A1179
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable serine-type endopeptidase inhibitor activity. Predicted to be involved in negative regulation of endopeptidase activity. Predicted to act upstream of or within several processes, including extracellular matrix organization; prostate gland morphogenesis; and regulation of epithelial cell proliferation. Located in cytoplasm. Biomarker of hepatocellular carcinoma.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 21-189 of human SERPINB5 (NP_002630.2).
SequenceEKEPLGNVLFSPICLSTSLSLAQVGAKGDTANEIGQVLHFENVKDVPFGFQTVTSDVNKLSSFYSLKLIKRLYVDKSLNLSTEFISSTKRPYAKELETVDFKDKLEETKGQINNSIKDLTDGHFENILADNSVNDQTKILVVNAAYFVGKWMKKFSESETKECPFRVNK
Gene ID
Swiss Prot
SynonymsPI5; maspin; B5
Calculated MW42kDa
Observed MW42kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa
Cellular locationSecreted, extracellular space.
Customer validation

WB(Mus musculus)

Co-IP(Homo sapiens)

IHC(Homo sapiens)

WB(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - [KO Validated] SERPINB5 Rabbit pAb (A1179)}

    Western blot - [KO Validated] SERPINB5 Rabbit pAb (A1179)

    Western blot analysis of various lysates using SERPINB5 Rabbit pAb (A1179) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    ABclonal:Western blot - [KO Validated] SERPINB5 Rabbit pAb (A1179)}

    Western blot - [KO Validated] SERPINB5 Rabbit pAb (A1179)

    Western blot analysis of lysates from wild type (WT) and SERPINB5 knockout (KO) HeLa cells, using [KO Validated] SERPINB5 Rabbit pAb (A1179) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 5s.
    ABclonal:Western blot - [KO Validated] SERPINB5 Rabbit pAb (A1179)}

    Western blot - [KO Validated] SERPINB5 Rabbit pAb (A1179)

    Western blot analysis of lysates from wild type(WT) and SERPINB5 knockout (KO) HeLa(KO) cells, using [KO Validated] SERPINB5 Rabbit pAb (A1179) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 5s.
    ABclonal:Immunohistochemistry - [KO Validated] SERPINB5 Rabbit pAb (A1179)}

    Immunohistochemistry - [KO Validated] SERPINB5 Rabbit pAb (A1179)

    Immunohistochemistry analysis of paraffin-embedded Human stomach using SERPINB5 Rabbit pAb (A1179) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunohistochemistry - [KO Validated] SERPINB5 Rabbit pAb (A1179)}

    Immunohistochemistry - [KO Validated] SERPINB5 Rabbit pAb (A1179)

    Immunohistochemistry analysis of paraffin-embedded Human rectum using SERPINB5 Rabbit pAb (A1179) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A1179? Please let us know so that we can cite the reference in this datasheet.