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[KO Validated] SIRT1 Rabbit pAb (A17307)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using SIRT1 Rabbit pAb (A17307) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Western blot analysis of lysates from 293T cells, using SIRT1 Rabbit pAb (A17307) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Western blot analysis of lysates from wild type (WT) and SIRT1 knockout (KO) HeLa cells using SIRT1 Rabbit pAb (A17307) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - [KO Validated] SIRT1 Rabbit pAb (A17307)

Immunofluorescence analysis of HeLa cells using SIRT1 Rabbit pAb (A17307) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using SIRT1 Rabbit pAb (A17307) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review

Overview

Product name	[KO Validated] SIRT1 Rabbit pAb
Catalog No.	A17307
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 448-747 of human SIRT1 (NP_036370.2).
Sequence	VALIPSSIPHEVPQILINREPLPHLHFDVELLGDCDVIINELCHRLGGEYAKLCCNPVKLSEITEKPPRTQKELAYLSELPPTPLHVSEDSSSPERTSPPDSSVIVTLLDQAAKSNDDLDVSESKGCMEEKPQEVQTSRNVESIAEQMENPDLKNVGSSTGEKNERTSVAGTVRKCWPNRVAKEQISRRLDGNQYLFLPPNRYIFHGAEVYSDSEDDVLSSSSCGSNSDSGTCQSPSLEEPMEDESEIEEFYNGLEDEPDVPERAGGAGFGTDGDDQEAINEAISVKQEVTDMNYPSNKS
Gene ID
Swiss Prot
Synonyms	SIR2; SIR2L1; SIR2alpha; SIRT1
Calculated MW	82kDa
Observed MW	120kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	293T, HeLa, Jurkat, NIH/3T3, C6, HeLa
Cellular location	Cytoplasm, Mitochondrion, Nucleus, Nucleus, PML body.
Customer validation	(HeLa cells) WB(Mus musculus, Homo sapiens, Rattus norvegicus, Other) IHC(Rattus norvegicus) IF(Mus musculus) WB(Mus musculus) IHC(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

[KO Validated] SIRT1 Rabbit pAb images

Western blot - [KO Validated] SIRT1 Rabbit pAb (A17307)

Immunofluorescence - [KO Validated] SIRT1 Rabbit pAb (A17307)

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

Product Protocols

References for [KO Validated] SIRT1 Rabbit pAb (A17307)

Product:

[KO Validated] SIRT1 Rabbit pAb

Journal:

Ecotoxicology and environmental safety

Application:

IF:

7.12

Species:

Mus musculus

PMID:

36399995

Title:

Puerarin prevents cadmium-induced mitochondrial fission in AML-12 cells via Sirt1-dependent pathway

References for [KO Validated] SIRT1 Rabbit pAb (A17307)

Product:

[KO Validated] SIRT1 Rabbit pAb

Journal:

Drug design, development and therapy

Application:

IHC

IF:

4.31

Species:

Rattus norvegicus

PMID:

36712944

Title:

A Novel Approach Based on Gut Microbiota Analysis and Network Pharmacology to Explain the Mechanisms of Action of Cichorium intybus L. Formula in the Improvement of Hyperuricemic Nephropathy in Rats - PMC

References for [KO Validated] SIRT1 Rabbit pAb (A17307)

Product:

[KO Validated] SIRT1 Rabbit pAb

Journal:

Frontiers in genetics

Application:

IF:

4.77

Species:

Homo sapiens

PMID:

36568393

Title:

Constructing a signature based on the SIRT family to help the prognosis and treatment sensitivity in glioma patients - PMC

References for [KO Validated] SIRT1 Rabbit pAb (A17307)

Product:

[KO Validated] SIRT1 Rabbit pAb

Journal:

Heliyon

Application:

IF:

3.77

Species:

Homo sapiens

PMID:

36950610

Title:

Danlou tablet inhibits high-glucose-induced cardiomyocyte apoptosis via the miR-34a-SIRT1 axis - PMC

References for [KO Validated] SIRT1 Rabbit pAb (A17307)

Product:

[KO Validated] SIRT1 Rabbit pAb

Journal:

Molecular nutrition & food research

Application:

IF:

6.57

Species:

Mus musculus

PMID:

36989169

Title:

Farnesol exerts protective effects against chronic sleep deprivation‐induced cognitive impairment via activation SIRT1/Nrf2 pathway in the hippocampi of adult mice

References for [KO Validated] SIRT1 Rabbit pAb (A17307)

Product:

[KO Validated] SIRT1 Rabbit pAb

Journal:

Cell reports

Application:

IF:

Species:

Mus musculus

PMID:

37393616

Title:

HIGD1A links SIRT1 activity to adipose browning by inhibiting the ROS/DNA damage pathway

References for [KO Validated] SIRT1 Rabbit pAb (A17307)

Product:

[KO Validated] SIRT1 Rabbit pAb

Journal:

Journal of hazardous materials

Application:

IF, WB

IF:

13.6

Species:

Mus musculus

PMID:

38401214

Title:

Low-Dose Deoxynivalenol Exposure Inhibits Hepatic Mitophagy and Hesperidin Reverses This Phenomenon by Activating SIRT1

References for [KO Validated] SIRT1 Rabbit pAb (A17307)

Product:

[KO Validated] SIRT1 Rabbit pAb

Journal:

Aging

Application:

IF:

5.2

Species:

Mus musculus

PMID:

38466649

Title:

Antagomir of miR-31-5p modulates macrophage polarization via the AMPK/SIRT1/NLRP3 signaling pathway to protect against DSS-induced colitis in mice - PMC

References for [KO Validated] SIRT1 Rabbit pAb (A17307)

Product:

[KO Validated] SIRT1 Rabbit pAb

Journal:

Aging

Application:

IF, IHC

IF:

5.2

Species:

Mus musculus

PMID:

38466649

Title:

Antagomir of miR-31-5p modulates macrophage polarization via the AMPK/SIRT1/NLRP3 signaling pathway to protect against DSS-induced colitis in mice - PMC

References for [KO Validated] SIRT1 Rabbit pAb (A17307)

Product:

[KO Validated] SIRT1 Rabbit pAb

Journal:

Journal of ethnopharmacology

Application:

IF:

5.19

Species:

Rattus norvegicus

PMID:

37741473