Western blot - [KO Validated] STAT5B Rabbit pAb (A21613)
Western blot analysis of various lysates using [KO Validated] STAT5B Rabbit pAb (A21613) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Western blot - [KO Validated] STAT5B Rabbit pAb (A21613)
Western blot analysis of lysates from wild type (WT) and STAT5B knockout (KO) HeLa cells, using [KO Validated] STAT5B Rabbit pAb (A21613) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Immunohistochemistry - [KO Validated] STAT5B Rabbit pAb (A21613)
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using [KO Validated] STAT5B Rabbit pAb (A21613) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - [KO Validated] STAT5B Rabbit pAb (A21613)
Immunohistochemistry analysis of paraffin-embedded Human tonsil using [KO Validated] STAT5B Rabbit pAb (A21613) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - [KO Validated] STAT5B Rabbit pAb (A21613)
Immunohistochemistry analysis of paraffin-embedded Mouse intestin using [KO Validated] STAT5B Rabbit pAb (A21613) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - [KO Validated] STAT5B Rabbit pAb (A21613)
Immunohistochemistry analysis of paraffin-embedded Mouse kidney using [KO Validated] STAT5B Rabbit pAb (A21613) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - [KO Validated] STAT5B Rabbit pAb (A21613)
Immunohistochemistry analysis of paraffin-embedded Rat intestine using [KO Validated] STAT5B Rabbit pAb (A21613) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunofluorescence - [KO Validated] STAT5B Rabbit pAb (A21613)
Immunofluorescence analysis of NIH/3T3 cells using STAT5B Rabbit pAb (A21613) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence - [KO Validated] STAT5B Rabbit pAb (A21613)
Immunofluorescence analysis of PC-12 cells using STAT5B Rabbit pAb (A21613) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence - [KO Validated] STAT5B Rabbit pAb (A21613)
Immunofluorescence analysis of U2OS cells using STAT5B Rabbit pAb (A21613) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation - [KO Validated] STAT5B Rabbit pAb (A21613)
Immunoprecipitation analysis of 600 μg extracts of Mouse thymus cells using 3 μg STAT5B antibody (A21613). Western blot was performed from the immunoprecipitate using STAT5B antibody (A21613) at a dilution of 1:1000.