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[KO Validated] Smad4 Rabbit mAb (A19116)

KO/KDValidated

Publications (10) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal: - [KO Validated] Smad4 Rabbit mAb (A19116)

CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for illumina (RK20265) from 10⁵ K562 cells with 1ug μg of [KO Validated] Smad4 Rabbit mAb (A19116), followed by incubation with Goat Anti-Rabbit IgG(H+L)(AS070). The CUT&Tag results denote the enrichment pattern of [KO Validated] Smad4 Rabbit mAb across chromosome 19 (upper panel) and the genomic region encompassing JUNB, a representative gene enriched in [KO Validated] Smad4 Rabbit mAb (lower panel).

ABclonal: - [KO Validated] Smad4 Rabbit mAb (A19116)

CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from10⁵ K562 with 1 μg of [KO Validated] Smad4 Rabbit mAb (A19116), followed by incubation with Goat Anti-Rabbit IgG(H+L)(AS070).The results denote the enrichment pattern of Smad4 around JUNB gene.

ABclonal:Western blot - [KO Validated] Smad4 Rabbit mAb (A19116)

Western blot analysis of various lysates using [KO Validated] Smad4 Rabbit mAb (A19116) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Western blot - [KO Validated] Smad4 Rabbit mAb (A19116)

Western blot analysis of lysates from wild type (WT) and Smad4 knockout (KO) 293T cells using [KO Validated] Smad4 Rabbit mAb (A19116) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunohistochemistry - [KO Validated] Smad4 Rabbit mAb (A19116)

Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using [KO Validated] Smad4 Rabbit mAb (A19116) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KO Validated] Smad4 Rabbit mAb (A19116)

Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using [KO Validated] Smad4 Rabbit mAb (A19116) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KO Validated] Smad4 Rabbit mAb (A19116)

Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using [KO Validated] Smad4 Rabbit mAb (A19116) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - [KO Validated] Smad4 Rabbit mAb (A19116)

Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using [KO Validated] Smad4 Rabbit mAb (A19116) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunoprecipitation - [KO Validated] Smad4 Rabbit mAb (A19116)

Immunoprecipitation analysis of 300 μg extracts of 293T cells using 3 μg Smad4 antibody (A19116). Western blot was performed from the immunoprecipitate using Smad4 antibody (A19116) at a dilution of1:1000.

ABclonal:Chromatin Immunoprecipitation - [KO Validated] Smad4 Rabbit mAb (A19116)

Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using [KO Validated] Smad4 Rabbit mAb (A19116) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Overview

Product name[KO Validated] Smad4 Rabbit mAb
Catalog No.A19116
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC5009-06
This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to transforming growth factor (TGF)-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The protein acts as a tumor suppressor and inhibits epithelial cell proliferation. It may also have an inhibitory effect on tumors by reducing angiogenesis and increasing blood vessel hyperpermeability. The encoded protein is a crucial component of the bone morphogenetic protein signaling pathway. The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 320-552 of human Smad4 (NP_005350.1).
SequencePEYWCSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLSAAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQPLD
Gene ID
Swiss Prot
SynonymsJIP; DPC4; MADH4; MYHRS; d4
Calculated MW60kDa
Observed MW70kDa/
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:6000
  • IHC-P 1:500 - 1:2000
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
  • ChIP 5μg antibody for 10μg-15μg of Chromatin
  • CUT&Tag 10⁵ cells /1 μg
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunoprecipitation    
Positive samples293T, HCT 116, HepG2, C6, NIH/3T3, Mouse lung, HCT 116, Hep G2, NIH/3T3, C6, Mouse lung, 293T
Cellular locationCytoplasm, Nucleus.
Customer validation

WB(Homo sapiens, Mus musculus)

Co-IP(Homo sapiens)

WB(Homo sapiens)

IHC(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal: - [KO Validated] Smad4 Rabbit mAb (A19116)}

- [KO Validated] Smad4 Rabbit mAb (A19116)

CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for illumina (RK20265) from 10⁵ K562 cells with 1ug μg of [KO Validated] Smad4 Rabbit mAb (A19116), followed by incubation with Goat Anti-Rabbit IgG(H+L)(AS070). The CUT&Tag results denote the enrichment pattern of [KO Validated] Smad4 Rabbit mAb across chromosome 19 (upper panel) and the genomic region encompassing JUNB, a representative gene enriched in [KO Validated] Smad4 Rabbit mAb (lower panel).
ABclonal: - [KO Validated] Smad4 Rabbit mAb (A19116)}

- [KO Validated] Smad4 Rabbit mAb (A19116)

CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from10⁵ K562 with 1 μg of [KO Validated] Smad4 Rabbit mAb (A19116), followed by incubation with Goat Anti-Rabbit IgG(H+L)(AS070).The results denote the enrichment pattern of Smad4 around JUNB gene.
ABclonal:Western blot - [KO Validated] Smad4 Rabbit mAb (A19116)}

Western blot - [KO Validated] Smad4 Rabbit mAb (A19116)

Western blot analysis of various lysates using [KO Validated] Smad4 Rabbit mAb (A19116) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Western blot - [KO Validated] Smad4 Rabbit mAb (A19116)}

Western blot - [KO Validated] Smad4 Rabbit mAb (A19116)

Western blot analysis of lysates from wild type (WT) and Smad4 knockout (KO) 293T cells using [KO Validated] Smad4 Rabbit mAb (A19116) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunohistochemistry - [KO Validated] Smad4 Rabbit mAb (A19116)}

Immunohistochemistry - [KO Validated] Smad4 Rabbit mAb (A19116)

Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using [KO Validated] Smad4 Rabbit mAb (A19116) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KO Validated] Smad4 Rabbit mAb (A19116)}

Immunohistochemistry - [KO Validated] Smad4 Rabbit mAb (A19116)

Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using [KO Validated] Smad4 Rabbit mAb (A19116) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KO Validated] Smad4 Rabbit mAb (A19116)}

Immunohistochemistry - [KO Validated] Smad4 Rabbit mAb (A19116)

Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using [KO Validated] Smad4 Rabbit mAb (A19116) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - [KO Validated] Smad4 Rabbit mAb (A19116)}

Immunohistochemistry - [KO Validated] Smad4 Rabbit mAb (A19116)

Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using [KO Validated] Smad4 Rabbit mAb (A19116) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunoprecipitation - [KO Validated] Smad4 Rabbit mAb (A19116)}

Immunoprecipitation - [KO Validated] Smad4 Rabbit mAb (A19116)

Immunoprecipitation analysis of 300 μg extracts of 293T cells using 3 μg Smad4 antibody (A19116). Western blot was performed from the immunoprecipitate using Smad4 antibody (A19116) at a dilution of1:1000.
ABclonal:Chromatin Immunoprecipitation - [KO Validated] Smad4 Rabbit mAb (A19116)}

Chromatin Immunoprecipitation - [KO Validated] Smad4 Rabbit mAb (A19116)

Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using [KO Validated] Smad4 Rabbit mAb (A19116) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A19116? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

Smad4 Rabbit pAb
A5657

WBIHC-PIF/ICCELISA

Reactivity:Human, Mouse, Rat

Smad4 Rabbit pAb

Secondary Antibodies (26)

HRP-conjugated Goat anti-Rabbit IgG (H+L)
AS014

WBDBIHC-PELISA

HRP-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Goat anti-Rabbit IgG (H+L)
AS011

IF/ICCFC

FITC-conjugated Goat anti-Rabbit IgG (H+L)

Cy3-conjugated Goat anti-Rabbit IgG (H+L)
AS007

IF/ICCFC

Cy3-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS053

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain
AS061

WBIP

HRP-conjugated Mouse anti-Rabbit IgG Light Chain

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain
AS063

WB

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)
AS039

IF/ICCFC

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)
AS040

IHC-PIF/ICCFC

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)
AS060

IF/ICCFC

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)

Goat Anti-Rabbit IgG(H+L)
AS070

WBELISACUT&Tag

Goat Anti-Rabbit IgG(H+L)

HRP-conjugated Donkey anti-Rabbit IgG (H+L)
AS038

WBIHC-P

HRP-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)
AS035

IF/ICCFC

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS073

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)
AS058

IF/ICCFC

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Donkey anti-Rabbit IgG (H+L)
AS042

IF/ICCFC

FITC-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)
AS056

IF/ICCFC

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)

AP-conjugated Goat anti-Rabbit IgG (H+L)
AS009

WB

AP-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG (H+L)
AS079

WB

HRP-conjugated Mouse anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb
AS126

IPELISA

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb

PE Mouse anti-Rabbit IgG, light chain specific mAb
AS129

FC

PE Mouse anti-Rabbit IgG, light chain specific mAb

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb
AS122

WBELISA

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb

Rabbit Anti-Chicken IgY (H+L)
AS090

WB

Rabbit Anti-Chicken IgY (H+L)

Rabbit Anti-Chicken IgY Fab
AS088

WB

Rabbit Anti-Chicken IgY Fab

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific
AS083

IF/ICCFC

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific

Rabbit Anti-Chicken IgY (H+L)
AS082

WB

Rabbit Anti-Chicken IgY (H+L)

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)
AS072

IF/ICCFC

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)