제품 > 항체 > 다중클론항체(pAb)

[KO Validated] TDP2 Rabbit pAb (A21536)

KO/KDValidated

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - [KO Validated] TDP2 Rabbit pAb (A21536)

Western blot analysis of lysates from wild type(WT) and TDP2 knockout (KO) HeLa(KO) cells, using [KO Validated] TDP2 Rabbit pAb (A21536) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product name[KO Validated] TDP2 Rabbit pAb
Catalog No.A21536
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of a superfamily of divalent cation-dependent phosphodiesterases. The encoded protein associates with CD40, tumor necrosis factor (TNF) receptor-75 and TNF receptor associated factors (TRAFs), and inhibits nuclear factor-kappa-B activation. This protein has sequence and structural similarities with APE1 endonuclease, which is involved in both DNA repair and the activation of transcription factors.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human TDP2 (NP_057698.2).
SequenceMELGSCLEGGREAAEEEGEPEVKKRRLLCVEFASVASCDAAVAQCFLAENDWEMERALNSYFEPPVEESALERRPETISEPKTYVDLTNEETTDSTTSKISPSEDTQQENGSMFSLITWNIDGLDLNNLSERARGVCSYLALYSPDVIFLQEVIPPYYSYLKKRSSNYEIITGHEEGYFTAIMLKKSRVKLKSQEIIPFP
Gene ID
Swiss Prot
SynonymsEAP2; AD022; EAPII; TTRAP; hTDP2; dJ30M3.3; P2
Calculated MW41kDa
Observed MW41kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa
Cellular locationCytoplasm, Nucleus, PML body, nucleolus.

Documents

Certificate of Compliance

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ABclonal:Western blot - [KO Validated] TDP2 Rabbit pAb (A21536)}

Western blot - [KO Validated] TDP2 Rabbit pAb (A21536)

Western blot analysis of lysates from wild type(WT) and TDP2 knockout (KO) HeLa(KO) cells, using [KO Validated] TDP2 Rabbit pAb (A21536) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

* For research use only. Not for therapeutic or diagnostic purposes.