제품 > 항체 > 다중클론항체(pAb)

[KO Validated] TLK2 Rabbit pAb (A6770)

KO/KDValidated

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - [KO Validated] TLK2 Rabbit pAb (A6770)

Western blot analysis of various lysates using [KO Validated] TLK2 Rabbit pAb (A6770) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

ABclonal:Western blot - [KO Validated] TLK2 Rabbit pAb (A6770)

Western blot analysis of lysates from wild type (WT) and TLK2 knockout (KO) NIH/3T3 cells, using [KO Validated] TLK2 Rabbit pAb (A6770) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product name[KO Validated] TLK2 Rabbit pAb
Catalog No.A6770
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a nuclear serine/threonine kinase that was first identified in Arabidopsis. The encoded protein is thought to function in the regulation of chromatin assembly in the S phase of the cell cycle by regulating the levels of a histone H3/H4 chaperone. This protein is associated with double-strand break repair of DNA damage caused by radiation. Pseudogenes of this gene are present on chromosomes 10 and 17. Alternate splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human TLK2 (NP_006843.2).
SequenceMMEELHSLDPRRQELLEARFTGVGVSKGPLNSESSNQSLCSVGSLSDKEVETPEKKQNDQRNRKRKAEPYETSQGKGTPRGHKISDYFEFAGGSAPGTSPGRSVPPVARSSPQHSLSNPLPRRVEQPLYGLDGSAAKEATEEQSALPTLMSVMLAKPRLDTEQLAQRGAGLCFTFVSAQQNSPSSTGSGNTEHSCSSQKQISIQHRQTQSDLTIEKISAL
Gene ID
Swiss Prot
SynonymsHsHPK; MRD57; PKU-ALPHA; K2
Calculated MW88kDa
Observed MW87kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T, Mouse testis, NIH/3T3
Cellular locationCytoplasm, Nucleus, cytoskeleton, perinuclear region

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - [KO Validated] TLK2 Rabbit pAb (A6770)}

    Western blot - [KO Validated] TLK2 Rabbit pAb (A6770)

    Western blot analysis of various lysates using [KO Validated] TLK2 Rabbit pAb (A6770) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 90s.
    ABclonal:Western blot - [KO Validated] TLK2 Rabbit pAb (A6770)}

    Western blot - [KO Validated] TLK2 Rabbit pAb (A6770)

    Western blot analysis of lysates from wild type (WT) and TLK2 knockout (KO) NIH/3T3 cells, using [KO Validated] TLK2 Rabbit pAb (A6770) at 1:500 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)