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[KO Validated] TMED10 Rabbit pAb (A18090)

KO/KDValidated

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] TMED10 Rabbit pAb (A18090)

Western blot analysis of various lysates using TMED10 Rabbit pAb (A18090) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - [KO Validated] TMED10 Rabbit pAb (A18090)

Western blot analysis of lysates from wild type (WT) and TMED10 knockout (KO) 293T cells, using [KO Validated] TMED10 Rabbit pAb (A18090) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - [KO Validated] TMED10 Rabbit pAb (A18090)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using TMED10 Rabbit pAb (A18090) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - [KO Validated] TMED10 Rabbit pAb (A18090)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using TMED10 Rabbit pAb (A18090) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - [KO Validated] TMED10 Rabbit pAb (A18090)

Immunohistochemistry analysis of paraffin-embedded Human liver damage using TMED10 Rabbit pAb (A18090) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - [KO Validated] TMED10 Rabbit pAb (A18090)

Immunofluorescence analysis of NIH-3T3 cells using TMED10 Rabbit pAb (A18090) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name[KO Validated] TMED10 Rabbit pAb
Catalog No.A18090
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a member of the EMP24/GP25L/p24 family and encodes a protein with a GOLD domain. This type I membrane protein is localized to the plasma membrane and golgi cisternae and is involved in vesicular protein trafficking. The protein is also a member of a heteromeric secretase complex and regulates the complex's gamma-secretase activity without affecting its epsilon-secretase activity. Mutations in this gene have been associated with early-onset familial Alzheimer's disease. This gene has a pseudogene on chromosome 8.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 35-190 of human TMED10 (NP_006818.3).
SequenceHLPINSRKCLREEIHKDLLVTGAYEISDQSGGAGGLRSHLKITDSAGHILYSKEDATKGKFAFTTEDYDMFEVCFESKGTGRIPDQLVILDMKHGVEAKNYEEIAKVEKLKPLEVELRRLEDLSESIVNDFAYMKKREEEMRDTNESTNTRVLYFS
Gene ID
Swiss Prot
Synonymsp23; TMP21; p24d1; S31I125; Tmp-21-I; S31III125; P24(DELTA); 10
Calculated MW25kDa
Observed MW21kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T
Cellular locationCell membrane, Cytoplasmic vesicle, Endoplasmic reticulum membrane, Endoplasmic reticulum-Golgi intermediate compartment membrane, Golgi apparatus, Melanosome, Single-pass type I membrane protein, cis-Golgi network membrane, secretory vesicle membrane, trans-Golgi.
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - [KO Validated] TMED10 Rabbit pAb (A18090)}

Western blot - [KO Validated] TMED10 Rabbit pAb (A18090)

Western blot analysis of various lysates using TMED10 Rabbit pAb (A18090) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - [KO Validated] TMED10 Rabbit pAb (A18090)}

Western blot - [KO Validated] TMED10 Rabbit pAb (A18090)

Western blot analysis of lysates from wild type (WT) and TMED10 knockout (KO) 293T cells, using [KO Validated] TMED10 Rabbit pAb (A18090) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - [KO Validated] TMED10 Rabbit pAb (A18090)}

Immunohistochemistry - [KO Validated] TMED10 Rabbit pAb (A18090)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using TMED10 Rabbit pAb (A18090) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - [KO Validated] TMED10 Rabbit pAb (A18090)}

Immunohistochemistry - [KO Validated] TMED10 Rabbit pAb (A18090)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using TMED10 Rabbit pAb (A18090) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - [KO Validated] TMED10 Rabbit pAb (A18090)}

Immunohistochemistry - [KO Validated] TMED10 Rabbit pAb (A18090)

Immunohistochemistry analysis of paraffin-embedded Human liver damage using TMED10 Rabbit pAb (A18090) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunofluorescence - [KO Validated] TMED10 Rabbit pAb (A18090)}

Immunofluorescence - [KO Validated] TMED10 Rabbit pAb (A18090)

Immunofluorescence analysis of NIH-3T3 cells using TMED10 Rabbit pAb (A18090) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)