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[KO Validated] TXLNA Rabbit pAb (A19889)

KO/KDValidated

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - [KO Validated] TXLNA Rabbit pAb (A19889)

Western blot analysis of lysates from wild type (WT) and TXLNA knockout (KO) HeLa cells, using [KO Validated] TXLNA Rabbit pAb (A19889) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product name[KO Validated] TXLNA Rabbit pAb
Catalog No.A19889
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable syntaxin binding activity. Predicted to be involved in exocytosis. Predicted to act upstream of or within B cell activation. Located in cytoplasm.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human TXLNA (NP_787048.1).
SequenceMKNQDKKNGAAKQSNPKSSPGQPEAGPEGAQERPSQAAPAVEAEGPGSSQAPRKPEGAQARTAQSGALRDVSEELSRQLEDILSTYCVDNNQGGPGEDGAQGEPAEPEDAEKSRTYVARNGEPEPTPVVNGEKEPSKGDPNTEEIRQSDEVGDRDHRRPQ
Gene ID
Swiss Prot
SynonymsIL14; TXLN; NA
Calculated MW62kDa
Observed MW80kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa
Cellular locationcytoplasm, cytosol, extracellular region
ABclonal:Western blot - [KO Validated] TXLNA Rabbit pAb (A19889)}

Western blot - [KO Validated] TXLNA Rabbit pAb (A19889)

Western blot analysis of lysates from wild type (WT) and TXLNA knockout (KO) HeLa cells, using [KO Validated] TXLNA Rabbit pAb (A19889) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (2)

Secondary Antibodies (26)