Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of various lysates using [KO Validated] UBE2O Rabbit pAb (A10036) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Western blot analysis of lysates from wild type (WT) and UBE2O knockout (KO) HeLa cells, using [KO Validated] UBE2O Rabbit pAb (A10036) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
Immunofluorescence analysis of HeLa cells using [KO Validated] UBE2O Rabbit pAb (A10036) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
| Product name | [KO Validated] UBE2O Rabbit pAb |
|---|---|
| Catalog No. | A10036 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1073-1292 of human UBE2O (NP_071349.3). |
|---|---|
| Sequence | LVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSTGGWRLVNRIESWLETHALLEKAQALPNGVPKASSSPEPPAVAELSDSGQQEPEDGGPAPGEASQGSDSEGGAQGLASASRDHTDQTSETAPDASVPPSVKPKKRRKSYRSFLPEKSGYPDIGFPLFPLSKGFIKSIRGVLTQFRAALLEAGMPECTEDK |
| Gene ID | |
| Swiss Prot | |
| Synonyms | E2-230K; 2O |
| Calculated MW | 141kDa |
| Observed MW | 200kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunofluorescence Immunoprecipitation |
| Positive samples | A-431, HepG2, Jurkat, Mouse heart, Mouse brain |
| Cellular location | Cytoplasm, Nucleus |
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* For research use only. Not for therapeutic or diagnostic purposes.
![ABclonal:Western blot - [KO Validated] UBE2O Rabbit pAb (A10036)](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A10036_P7328_WB_01.jpg?t=1702600752 "ABclonal:Western blot - [KO Validated] UBE2O Rabbit pAb (A10036)")
![ABclonal:Western blot - [KO Validated] UBE2O Rabbit pAb (A10036)](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A10036_P7328_KO-WB_01.jpg?t=1702600752 "ABclonal:Western blot - [KO Validated] UBE2O Rabbit pAb (A10036)")
![ABclonal:Immunofluorescence - [KO Validated] UBE2O Rabbit pAb (A10036)](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A10036_P7328_IF_01.jpg?t=1702600752 "ABclonal:Immunofluorescence - [KO Validated] UBE2O Rabbit pAb (A10036)")
![ABclonal:Immunoprecipitation - [KO Validated] UBE2O Rabbit pAb (A10036)](https://abclonal.oss-cn-hangzhou.aliyuncs.com/A10036_P7328_IP_01.jpg?t=1702600752 "ABclonal:Immunoprecipitation - [KO Validated] UBE2O Rabbit pAb (A10036)")
