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KPNA5 Rabbit pAb (A7331)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - KPNA5 Rabbit pAb (A7331)

Western blot analysis of extracts of various cell lines, using KPNA5 antibody (A7331) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 20s.

Overview

Product nameKPNA5 Rabbit pAb
Catalog No.A7331
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC) which consists of 60-100 proteins and is probably 120 million daltons in molecular size. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion; larger molecules are transported by an active process. Most nuclear proteins contain short basic amino acid sequences known as nuclear localization signals (NLSs). KPNA5 protein belongs to the importin alpha protein family and is thought to be involved in NLS-dependent protein import into the nucleus.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human KPNA5 (NP_002260.2).
SequenceMDAMASPGKDNYRMKSYKNKALNPQEMRRRREEEGIQLRKQKREEQLFKRRNVYLPRNDESMLESPIQDPDISSTVPIPEEEVVTTDMVQMIFSNNADQQLTATQKFRKLLSKEPNPPIDQVIQKPGVVQRFVKFLERNENCTLQFEAAWALTNIASGTFLHTKVVIETGAVPIFIKLLNSEHEDVQEQAVWALGNIAGDNAECRDFVLNCEILPPLLELLTNSNRLTTTRNAVWALSNL
Gene ID
Swiss Prot
SynonymsSRP6; IPOA6
Calculated MW61kDa
Observed MW60kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesJurkat, SKOV3, HepG2, NCI-H460, Mouse testis, Mouse liver, Mouse heart, Rat liver
Cellular locationCytoplasm
Customer validation

WB(Homo sapiens)

Co-IP(Homo sapiens)

Documents

Certificate of Compliance

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    ABclonal:Western blot - KPNA5 Rabbit pAb (A7331)}

    Western blot - KPNA5 Rabbit pAb (A7331)

    Western blot analysis of extracts of various cell lines, using KPNA5 antibody (A7331) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 20s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A7331? Please let us know so that we can cite the reference in this datasheet.

    Secondary Antibodies (26)