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KPNA6 Rabbit PolymAb® (A25199PM)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - KPNA6 Rabbit PolymAb® (A25199PM)

Western blot analysis of lysates from HeLa cells using KPNA6 Rabbit PolymAb® (A25199PM) at 1:20000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Immunohistochemistry - KPNA6 Rabbit PolymAb® (A25199PM)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using KPNA6 Rabbit PolymAb® (A25199PM) at a dilution of 1:3000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - KPNA6 Rabbit PolymAb® (A25199PM)

Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using KPNA6 Rabbit PolymAb® (A25199PM) at a dilution of 1:3000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - KPNA6 Rabbit PolymAb® (A25199PM)

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using KPNA6 Rabbit PolymAb® (A25199PM) at a dilution of 1:3000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - KPNA6 Rabbit PolymAb® (A25199PM)

Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using KPNA6 Rabbit PolymAb® (A25199PM) at a dilution of 1:3000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - KPNA6 Rabbit PolymAb® (A25199PM)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using KPNA6 Rabbit PolymAb® (A25199PM) at a dilution of 1:3000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - KPNA6 Rabbit PolymAb® (A25199PM)

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using KPNA6 Rabbit PolymAb® (A25199PM) at a dilution of 1:3000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - KPNA6 Rabbit PolymAb® (A25199PM)

Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using KPNA6 Rabbit PolymAb® (A25199PM) at a dilution of 1:3000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - KPNA6 Rabbit PolymAb® (A25199PM)

Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using KPNA6 Rabbit PolymAb® (A25199PM) at a dilution of 1:3000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - KPNA6 Rabbit PolymAb® (A25199PM)

Confocal imaging of A-431 cells using KPNA6 Rabbit PolymAb® (A25199PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - KPNA6 Rabbit PolymAb® (A25199PM)

Confocal imaging of HeLa cells using KPNA6 Rabbit PolymAb® (A25199PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - KPNA6 Rabbit PolymAb® (A25199PM)

Confocal imaging of NIH/3T3 cells using KPNA6 Rabbit PolymAb® (A25199PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Overview

Product nameKPNA6 Rabbit PolymAb®
Catalog No.A25199PM
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. The protein encoded by this gene is a member of the importin alpha family.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 69-169 of human KPNA6 (NP_036448.1).
SequenceDSYVSSTTGESVITREMVEMLFSDDSDLQLATTQKFRKLLSKEPSPPIDEVINTPRVVDRFVEFLKRNENCTLQFEAAWALTNIASGTSQQTKIVIEAGAV
Gene ID
Swiss Prot
SynonymsIPOA7; KPNA6
Calculated MW60kDa
Observed MW60kDa/
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:3000 - 1:20000
  • IHC-P 1:1000-1:5000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa
Cellular locationcytosol, nucleoplasm, nucleus.
    ABclonal:Western blot - KPNA6 Rabbit PolymAb® (A25199PM)}

    Western blot - KPNA6 Rabbit PolymAb® (A25199PM)

    Western blot analysis of lysates from HeLa cells using KPNA6 Rabbit PolymAb® (A25199PM) at 1:20000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 20s.
    ABclonal:Immunohistochemistry - KPNA6 Rabbit PolymAb® (A25199PM)}

    Immunohistochemistry - KPNA6 Rabbit PolymAb® (A25199PM)

    Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using KPNA6 Rabbit PolymAb® (A25199PM) at a dilution of 1:3000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - KPNA6 Rabbit PolymAb® (A25199PM)}

    Immunohistochemistry - KPNA6 Rabbit PolymAb® (A25199PM)

    Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using KPNA6 Rabbit PolymAb® (A25199PM) at a dilution of 1:3000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - KPNA6 Rabbit PolymAb® (A25199PM)}

    Immunohistochemistry - KPNA6 Rabbit PolymAb® (A25199PM)

    Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using KPNA6 Rabbit PolymAb® (A25199PM) at a dilution of 1:3000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - KPNA6 Rabbit PolymAb® (A25199PM)}

    Immunohistochemistry - KPNA6 Rabbit PolymAb® (A25199PM)

    Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using KPNA6 Rabbit PolymAb® (A25199PM) at a dilution of 1:3000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - KPNA6 Rabbit PolymAb® (A25199PM)}

    Immunohistochemistry - KPNA6 Rabbit PolymAb® (A25199PM)

    Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using KPNA6 Rabbit PolymAb® (A25199PM) at a dilution of 1:3000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - KPNA6 Rabbit PolymAb® (A25199PM)}

    Immunohistochemistry - KPNA6 Rabbit PolymAb® (A25199PM)

    Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using KPNA6 Rabbit PolymAb® (A25199PM) at a dilution of 1:3000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - KPNA6 Rabbit PolymAb® (A25199PM)}

    Immunohistochemistry - KPNA6 Rabbit PolymAb® (A25199PM)

    Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using KPNA6 Rabbit PolymAb® (A25199PM) at a dilution of 1:3000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - KPNA6 Rabbit PolymAb® (A25199PM)}

    Immunohistochemistry - KPNA6 Rabbit PolymAb® (A25199PM)

    Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using KPNA6 Rabbit PolymAb® (A25199PM) at a dilution of 1:3000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunofluorescence - KPNA6 Rabbit PolymAb® (A25199PM)}

    Immunofluorescence - KPNA6 Rabbit PolymAb® (A25199PM)

    Confocal imaging of A-431 cells using KPNA6 Rabbit PolymAb® (A25199PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
    ABclonal:Immunofluorescence - KPNA6 Rabbit PolymAb® (A25199PM)}

    Immunofluorescence - KPNA6 Rabbit PolymAb® (A25199PM)

    Confocal imaging of HeLa cells using KPNA6 Rabbit PolymAb® (A25199PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
    ABclonal:Immunofluorescence - KPNA6 Rabbit PolymAb® (A25199PM)}

    Immunofluorescence - KPNA6 Rabbit PolymAb® (A25199PM)

    Confocal imaging of NIH/3T3 cells using KPNA6 Rabbit PolymAb® (A25199PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

    * For research use only. Not for therapeutic or diagnostic purposes.

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