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KRAS+HRAS+NRAS Rabbit mAb (A19779)

Publications (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - KRAS+HRAS+NRAS Rabbit mAb (A19779)

Western blot analysis of various lysates using KRAS+HRAS+NRAS Rabbit mAb (A19779) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - KRAS+HRAS+NRAS Rabbit mAb (A19779)

Western blot analysis of various lysates using KRAS+HRAS+NRAS Rabbit mAb (A19779) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - KRAS+HRAS+NRAS Rabbit mAb (A19779)

Immunofluorescence analysis of NIH-3T3 cells using KRAS+HRAS+NRAS Rabbit mAb (A19779) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameKRAS+HRAS+NRAS Rabbit mAb
Catalog No.A19779
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2369
This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008]
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-188 of human NRAS (P01111).
SequenceMTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNSKSFADINLYREQIKRVKDSDDVPMVLVGNKCDLPTRTVDTKQAHELAKSYGIPFIETSAKTRQGVEDAFYTLVREIRQYRMKKLNSSDDGTQGCMGLPCVV
Gene ID
Swiss Prot
SynonymsC-K-RAS; CFC2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KI-RAS; KRAS1; KRAS2; NS; NS3; RALD; RASK2; c-Ki-ras2; KRAS+HRAS+NRAS
Calculated MW21kDa
Observed MW21kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • IF/ICC 1:100 - 1:400
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHT-29, 293T, MCF7, NIH/3T3, C6, Mouse lung, Mouse brain, Rat brain
Cellular locationcytosol, extracellular exosome, Golgi apparatus, plasma membrane.
Customer validation

WB(Homo sapiens, Sus scrofa)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - KRAS+HRAS+NRAS Rabbit mAb (A19779)}

    Western blot - KRAS+HRAS+NRAS Rabbit mAb (A19779)

    Western blot analysis of various lysates using KRAS+HRAS+NRAS Rabbit mAb (A19779) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Western blot - KRAS+HRAS+NRAS Rabbit mAb (A19779)}

    Western blot - KRAS+HRAS+NRAS Rabbit mAb (A19779)

    Western blot analysis of various lysates using KRAS+HRAS+NRAS Rabbit mAb (A19779) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.
    ABclonal:Immunofluorescence - KRAS+HRAS+NRAS Rabbit mAb (A19779)}

    Immunofluorescence - KRAS+HRAS+NRAS Rabbit mAb (A19779)

    Immunofluorescence analysis of NIH-3T3 cells using KRAS+HRAS+NRAS Rabbit mAb (A19779) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A19779? Please let us know so that we can cite the reference in this datasheet.

    항체 (2)

    Secondary Antibodies (26)