제품 > 항체 > 다중클론항체(pAb)

KRT81 Rabbit pAb (A3940)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - KRT81 Rabbit pAb (A3940)

Western blot analysis of various lysates using KRT81 Rabbit pAb (A3940) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product nameKRT81 Rabbit pAb
Catalog No.A3940
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the keratin gene family. As a type II hair keratin, it is a basic protein which heterodimerizes with type I keratins to form hair and nails. The type II hair keratins are clustered in a region of chromosome 12q13 and are grouped into two distinct subfamilies based on structure similarity. One subfamily, consisting of KRTHB1, KRTHB3, and KRTHB6, is highly related. The other less-related subfamily includes KRTHB2, KRTHB4, and KRTHB5. All hair keratins are expressed in the hair follicle; this hair keratin, as well as KRTHB3 and KRTHB6, is found primarily in the hair cortex. Mutations in this gene and KRTHB6 have been observed in patients with a rare dominant hair disease, monilethrix.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 350-450 of human KRT81 (NP_002272.2).
SequenceVAQSEQQGEAALSDARCKLAELEGALQKAKQDMACLIREYQEVMNSKLGLDIEIATYRRLLEGEEQRLCEGIGAVNVCVSSSRGGVVCGDLCVSGSRPVTG
Gene ID
Swiss Prot
SynonymsHB1; K81; Hb-1; KRTHB1; MLN137; ghHkb1; hHAKB2-1; KRT81
Calculated MW55kDa
Observed MW55kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T, NCI-H460, MCF7, Mouse ovary, Mouse testis
Cellular locationcytosol, extracellular space
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - KRT81 Rabbit pAb (A3940)}

Western blot - KRT81 Rabbit pAb (A3940)

Western blot analysis of various lysates using KRT81 Rabbit pAb (A3940) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A3940? Please let us know so that we can cite the reference in this datasheet.

Secondary Antibodies (26)