제품 > 항체 > 다중클론항체(pAb)

KSR1 Rabbit pAb (A15118)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using KSR1 Rabbit pAb (A15118) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - KSR1 Rabbit pAb (A15118)

Immunofluorescence analysis of C6 cells using KSR1 Rabbit pAb (A15118) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	KSR1 Rabbit pAb
Catalog No.	A15118
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Enables 14-3-3 protein binding activity; ATP binding activity; and protein C-terminus binding activity. Involved in positive regulation of MAPK cascade. Located in endoplasmic reticulum and membrane. Part of protein-containing complex. Implicated in breast adenocarcinoma. Biomarker of breast cancer.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 320-420 of human KSR1 (NP_055053.1).
Sequence	SSPAPFPTSSNPSSATTPPNPSPGQRDSRFNFPAAYFIHHRQQFIFPVPSAGHCWKCLLIAESLKENAFNISAFAHAAPLPEAADGTRLDDQPKADVLEAH
Gene ID
Swiss Prot
Synonyms	KSR; RSU2; KSR1
Calculated MW	102kDa
Observed MW	110kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IF/ICC 1:50 - 1:100
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	U-251MG, HeLa, Jurkat, mouse brain, mouse spleen, mouse testis, mouse kidney, mouse lung, rat brain
Cellular location	Cytoplasm, Endoplasmic reticulum membrane, Membrane, Peripheral membrane protein