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Kallikrein 8 Rabbit mAb (A9409)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Kallikrein 8 Rabbit mAb (A9409)

Western blot analysis of various lysates using (A9409) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - Kallikrein 8 Rabbit mAb (A9409)

Western blot analysis of lysates from mouse uterus, using (A9409) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Kallikrein 8 Rabbit mAb (A9409)

Immunohistochemistry analysis of Kallikrein 8 in paraffin-embedded human liver cancer tissue using Kallikrein 8 Rabbit mAb (A9409) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Kallikrein 8 Rabbit mAb (A9409)

Immunohistochemistry analysis of Kallikrein 8 in paraffin-embedded human colon carcinoma tissue using Kallikrein 8 Rabbit mAb (A9409) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Kallikrein 8 Rabbit mAb (A9409)

Immunohistochemistry analysis of Kallikrein 8 in paraffin-embedded human kidney tissue using Kallikrein 8 Rabbit mAb (A9409) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Overview

Product nameKallikrein 8 Rabbit mAb
Catalog No.A9409
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2716
Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in tandem in a gene cluster on chromosome 19. The encoded protein may be involved in proteolytic cascade in the skin and may serve as a biomarker for ovarian cancer. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 161-260 of human Kallikrein 8 (O60259).
SequenceTSPRENFPDTLNCAEVKIFPQKKCEDAYPGQITDGMVCAGSSKGADTCQGDSGGPLVCDGALQGITSWGSDPCGRSDKPGVYTNICRYLDWIKKIIGSKG
Gene ID
Swiss Prot
SynonymsNP; HNP; NRPN; PRSS19; TADG14; Kallikrein 8
Calculated MW28kDa
Observed MW35kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesBxPC-3, A-431, SH-SY5Y, Mouse uterus
Cellular locationCytoplasm, Secreted
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - Kallikrein 8 Rabbit mAb (A9409)}

Western blot - Kallikrein 8 Rabbit mAb (A9409)

Western blot analysis of various lysates using (A9409) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - Kallikrein 8 Rabbit mAb (A9409)}

Western blot - Kallikrein 8 Rabbit mAb (A9409)

Western blot analysis of lysates from mouse uterus, using (A9409) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.
ABclonal:Immunohistochemistry - Kallikrein 8 Rabbit mAb (A9409)}

Immunohistochemistry - Kallikrein 8 Rabbit mAb (A9409)

Immunohistochemistry analysis of Kallikrein 8 in paraffin-embedded human liver cancer tissue using Kallikrein 8 Rabbit mAb (A9409) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Kallikrein 8 Rabbit mAb (A9409)}

Immunohistochemistry - Kallikrein 8 Rabbit mAb (A9409)

Immunohistochemistry analysis of Kallikrein 8 in paraffin-embedded human colon carcinoma tissue using Kallikrein 8 Rabbit mAb (A9409) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Kallikrein 8 Rabbit mAb (A9409)}

Immunohistochemistry - Kallikrein 8 Rabbit mAb (A9409)

Immunohistochemistry analysis of Kallikrein 8 in paraffin-embedded human kidney tissue using Kallikrein 8 Rabbit mAb (A9409) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A9409? Please let us know so that we can cite the reference in this datasheet.

재조합 단백질 (1)

Secondary Antibodies (26)