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Karyopherin β3 (IPO5) Rabbit mAb (A22764)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Karyopherin β3 (IPO5) Rabbit mAb (A22764)

Western blot analysis of various lysates, using Karyopherin β3 (IPO5) Rabbit mAb (A22764) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product nameKaryopherin β3 (IPO5) Rabbit mAb
Catalog No.A22764
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC59432
Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 20-300 of human Karyopherin β3 (IPO5). (NP_002262.4).
SequenceSPDNVVRKQAEETYENIPGQSKITFLLQAIRNTTAAEEARQMAAVLLRRLLSSAFDEVYPALPSDVQTAIKSELLMIIQMETQSSMRKKVCDIAAELARNLIDEDGNNQWPEGLKFLFDSVSSQNVGLREAALHIFWNFPGIFGNQQQHYLDVIKRMLVQCMQDQEHPSIRTLSARATAAFILANEHNVALFKHFADLLPGFLQAVNDSCYQNDDSVLKSLVEIADTVPKYLRPHLEATLQLSLKLCGDTSLNNMQRQLALEVIVTLSETAAAMLRKHTNI
Gene ID
Swiss Prot
SynonymsIMB3; Pse1; imp5; KPNB3; RANBP5; Karyopherin β3 (IPO5)
Calculated MW124kDa
Observed MW124kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:10000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesDaudi, Mouse testis, Rat testis
Cellular locationCytoplasm, Nucleus, nucleolus
Customer validation

IHC(Sus scrofa)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Karyopherin β3 (IPO5) Rabbit mAb (A22764)}

    Western blot - Karyopherin β3 (IPO5) Rabbit mAb (A22764)

    Western blot analysis of various lysates, using Karyopherin β3 (IPO5) Rabbit mAb (A22764) at 1:10000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A22764? Please let us know so that we can cite the reference in this datasheet.

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