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Kinesin light chain 1 (KLC1) Rabbit mAb (A19249)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Kinesin light chain 1 (KLC1) Rabbit mAb (A19249)

Western blot analysis of extracts of various cell lines, using Kinesin light chain 1 (Kinesin light chain 1 (KLC1)) antibody (A19249) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - Kinesin light chain 1 (KLC1) Rabbit mAb (A19249)

Western blot analysis of extracts of Mouse brain, using Kinesin light chain 1 (Kinesin light chain 1 (KLC1)) antibody (A19249) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product nameKinesin light chain 1 (KLC1) Rabbit mAb
Catalog No.A19249
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2409
Conventional kinesin is a tetrameric molecule composed of two heavy chains and two light chains, and transports various cargos along microtubules toward their plus ends. The heavy chains provide the motor activity, while the light chains bind to various cargos. This gene encodes a member of the kinesin light chain family. It associates with kinesin heavy chain through an N-terminal domain, and six tetratricopeptide repeat (TPR) motifs are thought to be involved in binding of cargos such as vesicles, mitochondria, and the Golgi complex. Thus, kinesin light chains function as adapter molecules and not motors per se. Although previously named "kinesin 2", this gene is not a member of the kinesin-2 / kinesin heavy chain subfamily of kinesin motor proteins. Extensive alternative splicing produces isoforms with different C-termini that are proposed to bind to different cargos; however, the full-length nature and/or biological validity of most of these variants have not been determined.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 450-550 of human Kinesin light chain 1 (KLC1) (Q07866).
SequenceGGWYKACKVDSPTVTTTLKNLGALYRRQGKFEAAETLEEAAMRSRKQGLDNVHKQRVAEVLNDPENMEKRRSRESLNVDVVKYESGPDGGEEVSMSVEWNG
Gene ID
Swiss Prot
SynonymsKLC; KNS2; KNS2A
Calculated MW65kDa
Observed MW65kDa/75kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T, MCF7, U-87MG, Mouse brain, Rat brain
Cellular locationCell projection, Cytoplasm, Cytoplasmic vesicle, cytoskeleton, growth cone

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ABclonal:Western blot - Kinesin light chain 1 (KLC1) Rabbit mAb (A19249)}

Western blot - Kinesin light chain 1 (KLC1) Rabbit mAb (A19249)

Western blot analysis of extracts of various cell lines, using Kinesin light chain 1 (Kinesin light chain 1 (KLC1)) antibody (A19249) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - Kinesin light chain 1 (KLC1) Rabbit mAb (A19249)}

Western blot - Kinesin light chain 1 (KLC1) Rabbit mAb (A19249)

Western blot analysis of extracts of Mouse brain, using Kinesin light chain 1 (Kinesin light chain 1 (KLC1)) antibody (A19249) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

* For research use only. Not for therapeutic or diagnostic purposes.

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