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L3MBTL3 Rabbit pAb (A7289)

Review (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - L3MBTL3 Rabbit pAb (A7289)

Western blot analysis of lysates from Mouse lung, using L3MBTL3 Rabbit pAb (A7289) at 1:4000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - L3MBTL3 Rabbit pAb (A7289)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using L3MBTL3 Rabbit pAb (A7289) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - L3MBTL3 Rabbit pAb (A7289)

Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using L3MBTL3 Rabbit pAb (A7289) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Review
ABclonal: review for L3MBTL3 Rabbit pAb(A7289)

Overview

Product nameL3MBTL3 Rabbit pAb
Catalog No.A7289
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the malignant brain tumor (MBT) family of chromatin interacting transcriptional repressors. Members of this family function as methyl-lysine readers, which recognize methylated lysine residues on histone protein tails, and are associated with the repression of gene expression. The encoded protein may regulate hematopoiesis. Homozygous deletion of this gene has been observed in human patients with medulloblastoma.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-255 of human L3MBTL3 (NP_115814.1).
SequenceMTESASSTSGQEFDVFSVMDWKDGVGTLPGSDLKFRVNEFGALEVITDENEMENVKKATATTTWMVPTAQEAPTSPPSSRPVFPPAYWTSPPGCPTVFSEKTGMPFRLKDPVKVEGLQFCENCCQYGNVDECLSGGNYCSQNCARHIKDKDQKEERDVEEDNEEEDPKCSRKKKPKLSLKADTKEDGEERDDEMENKQDVRILRGSQRARRKRRGDSAVLKQGLPPKGKKAWCWASYLEEEKAVAVPAKLFKEHQ
Gene ID
Swiss Prot
SynonymsMBT1; MBT-1; L3MBTL3
Calculated MW88kDa
Observed MW100kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:4000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse lung
Cellular locationNucleus
Customer validation

(Mouse testis)

Documents

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ABclonal:Western blot - L3MBTL3 Rabbit pAb (A7289)}

Western blot - L3MBTL3 Rabbit pAb (A7289)

Western blot analysis of lysates from Mouse lung, using L3MBTL3 Rabbit pAb (A7289) at 1:4000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - L3MBTL3 Rabbit pAb (A7289)}

Immunohistochemistry - L3MBTL3 Rabbit pAb (A7289)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using L3MBTL3 Rabbit pAb (A7289) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - L3MBTL3 Rabbit pAb (A7289)}

Immunohistochemistry - L3MBTL3 Rabbit pAb (A7289)

Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using L3MBTL3 Rabbit pAb (A7289) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

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