제품 > 항체 > 다중클론항체(pAb)

LAGE3 Rabbit pAb (A20546)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - LAGE3 Rabbit pAb (A20546)

Western blot analysis of various lysates using LAGE3 Rabbit pAb (A20546) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product nameLAGE3 Rabbit pAb
Catalog No.A20546
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene belongs to the ESO/LAGE gene family, members of which are clustered together on chromosome Xq28, and have similar exon-intron structures. Unlike the other family members which are normally expressed only in testis and activated in a wide range of human tumors, this gene is ubiquitously expressed in somatic tissues. The latter, combined with the finding that it is highly conserved in mouse and rat, suggests that the encoded protein is functionally important. An intronless pseudogene with high sequence similarity to this gene is located on chromosome 9.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-143 of human LAGE3 (NP_006005.2).
SequenceMRDADADAGGGADGGDGRGGHSCRGGVDTAAAPAGGAPPAHAPGPGRDAASAARGSRMRPHIFTLSVPFPTPLEAEIAHGSLAPDAEPHQRVVGKDLTVSGRILVVRWKAEDCRLLRISVINFLDQLSLVVRTMQRFGPPVSR
Gene ID
Swiss Prot
SynonymsCVG5; ESO3; Pcc1; ITBA2; GAMOS2; DXS9879E; DXS9951E; LAGE3
Calculated MW15kDa
Observed MW17kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHepG2, NCI-H460, BxPC-3
Cellular locationcytoplasm, nuclear body, nucleoplasm, nucleus
    ABclonal:Western blot - LAGE3 Rabbit pAb (A20546)}

    Western blot - LAGE3 Rabbit pAb (A20546)

    Western blot analysis of various lysates using LAGE3 Rabbit pAb (A20546) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)