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LAMC1 Rabbit pAb (A16020)

Publications (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - LAMC1 Rabbit pAb (A16020)

Western blot analysis of various lysates using LAMC1 Rabbit pAb (A16020) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - LAMC1 Rabbit pAb (A16020)

Western blot analysis of lysates from LO2 cells, using LAMC1 Rabbit pAb (A16020) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

ABclonal:Immunohistochemistry - LAMC1 Rabbit pAb (A16020)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using LAMC1 Rabbit pAb (A16020) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - LAMC1 Rabbit pAb (A16020)

Immunohistochemistry analysis of paraffin-embedded Human uterus using LAMC1 Rabbit pAb (A16020) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - LAMC1 Rabbit pAb (A16020)

Immunohistochemistry analysis of paraffin-embedded Human uterine cancer using LAMC1 Rabbit pAb (A16020) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Overview

Product nameLAMC1 Rabbit pAb
Catalog No.A16020
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), have a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin, gamma 1. The gamma 1 chain, formerly thought to be a beta chain, contains structural domains similar to beta chains, however, lacks the short alpha region separating domains I and II. The structural organization of this gene also suggested that it had diverged considerably from the beta chain genes. Embryos of transgenic mice in which both alleles of the gamma 1 chain gene were inactivated by homologous recombination, lacked basement membranes, indicating that laminin, gamma 1 chain is necessary for laminin heterotrimer assembly. It has been inferred by analogy with the strikingly similar 3' UTR sequence in mouse laminin gamma 1 cDNA, that multiple polyadenylation sites are utilized in human to generate the 2 different sized mRNAs (5.5 and 7.5 kb) seen on Northern analysis.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1030-1275 of human LAMC1 (NP_002284.3).
SequenceECPACYRLVKDKVADHRVKLQELESLIANLGTGDEMVTDQAFEDRLKEAEREVMDLLREAQDVKDVDQNLMDRLQRVNNTLSSQISRLQNIRNTIEETGNLAEQARAHVENTERLIEIASRELEKAKVAAANVSVTQPESTGDPNNMTLLAEEARKLAERHKQEADDIVRVAKTANDTSTEAYNLLLRTLAGENQTAFEIEELNRKYEQAKNISQDLEKQAARVHEEAKRAGDKAVEIYASVAQLS
Gene ID
Swiss Prot
SynonymsLAMB2; LAMC1
Calculated MW178kDa
Observed MW220kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesLO2, Mouse heart, Mouse kidney, Rat heart
Cellular locationbasement membrane, endoplasmic reticulum lumen, extracellular exosome, extracellular region, extracellular space.
Customer validation

WB(Homo sapiens, Sus scrofa)

IHC(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - LAMC1 Rabbit pAb (A16020)}

    Western blot - LAMC1 Rabbit pAb (A16020)

    Western blot analysis of various lysates using LAMC1 Rabbit pAb (A16020) at 1:3000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Western blot - LAMC1 Rabbit pAb (A16020)}

    Western blot - LAMC1 Rabbit pAb (A16020)

    Western blot analysis of lysates from LO2 cells, using LAMC1 Rabbit pAb (A16020) at 1:3000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 30s.
    ABclonal:Immunohistochemistry - LAMC1 Rabbit pAb (A16020)}

    Immunohistochemistry - LAMC1 Rabbit pAb (A16020)

    Immunohistochemistry analysis of paraffin-embedded Human breast cancer using LAMC1 Rabbit pAb (A16020) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunohistochemistry - LAMC1 Rabbit pAb (A16020)}

    Immunohistochemistry - LAMC1 Rabbit pAb (A16020)

    Immunohistochemistry analysis of paraffin-embedded Human uterus using LAMC1 Rabbit pAb (A16020) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunohistochemistry - LAMC1 Rabbit pAb (A16020)}

    Immunohistochemistry - LAMC1 Rabbit pAb (A16020)

    Immunohistochemistry analysis of paraffin-embedded Human uterine cancer using LAMC1 Rabbit pAb (A16020) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A16020? Please let us know so that we can cite the reference in this datasheet.

    항체 (1)

    Secondary Antibodies (26)