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LCN1 Rabbit pAb (A9828)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - LCN1 Rabbit pAb (A9828)

Western blot analysis of various lysates using LCN1 Rabbit pAb (A9828) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - LCN1 Rabbit pAb (A9828)

Immunohistochemistry analysis of paraffin-embedded Human prostate using LCN1 Rabbit pAb (A9828) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameLCN1 Rabbit pAb
Catalog No.A9828
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the lipocalin family of small secretory proteins. Lipocalins are extracellular transport proteins that bind to a variety of hydrophobic ligands. The encoded protein is the primary lipid binding protein in tears and is overproduced in response to multiple stimuli including infection and stress. The encoded protein may be a marker for chromosome aneuploidy as well as an autoantigen in Sjogren's syndrome. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and two pseudogenes of this gene are also located on the long arm of chromosome 9.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 19-176 of human LCN1 (NP_002288.1).
SequenceHHLLASDEEIQDVSGTWYLKAMTVDREFPEMNLESVTPMTLTTLEGGNLEAKVTMLISGRCQEVKAVLEKTDEPGKYTADGGKHVAYIIRSHVKDHYIFYCEGELHGKPVRGVKLVGRDPKNNLEALEDFEKAAGARGLSTESILIPRQSETCSPGSD
Gene ID
Swiss Prot
SynonymsTP; TLC; PMFA; VEGP; LCN1
Calculated MW19kDa
Observed MW16kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHL-60, 293T
Cellular locationSecreted

Documents

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    ABclonal:Western blot - LCN1 Rabbit pAb (A9828)}

    Western blot - LCN1 Rabbit pAb (A9828)

    Western blot analysis of various lysates using LCN1 Rabbit pAb (A9828) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Immunohistochemistry - LCN1 Rabbit pAb (A9828)}

    Immunohistochemistry - LCN1 Rabbit pAb (A9828)

    Immunohistochemistry analysis of paraffin-embedded Human prostate using LCN1 Rabbit pAb (A9828) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.

    재조합 단백질 (1)

    Secondary Antibodies (26)