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LDB3 Rabbit pAb (A7462)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - LDB3 Rabbit pAb (A7462)

Western blot analysis of various lysates using LDB3 Rabbit pAb (A7462) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - LDB3 Rabbit pAb (A7462)

Immunohistochemistry analysis of paraffin-embedded Mouse stomach using LDB3 Rabbit pAb (A7462) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - LDB3 Rabbit pAb (A7462)

Immunofluorescence analysis of paraffin-embedded human placenta using LDB3 Rabbit pAb (A7462) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - LDB3 Rabbit pAb (A7462)

Immunofluorescence analysis of paraffin-embedded rat heart using LDB3 Rabbit pAb (A7462) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - LDB3 Rabbit pAb (A7462)

Immunofluorescence analysis of paraffin-embedded mouse heart using LDB3 Rabbit pAb (A7462) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameLDB3 Rabbit pAb
Catalog No.A7462
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a PDZ domain-containing protein. PDZ motifs are modular protein-protein interaction domains consisting of 80-120 amino acid residues. PDZ domain-containing proteins interact with each other in cytoskeletal assembly or with other proteins involved in targeting and clustering of membrane proteins. The protein encoded by this gene interacts with alpha-actinin-2 through its N-terminal PDZ domain and with protein kinase C via its C-terminal LIM domains. The LIM domain is a cysteine-rich motif defined by 50-60 amino acids containing two zinc-binding modules. This protein also interacts with all three members of the myozenin family. Mutations in this gene have been associated with myofibrillar myopathy and dilated cardiomyopathy. Alternatively spliced transcript variants encoding different isoforms have been identified; all isoforms have N-terminal PDZ domains while only longer isoforms (1, 2 and 5) have C-terminal LIM domains.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-283 of human LDB3 (NP_001073585.1).
SequenceMSYSVTLTGPGPWGFRLQGGKDFNMPLTISRITPGSKAAQSQLSQGDLVVAIDGVNTDTMTHLEAQNKIKSASYNLSLTLQKSKRPIPISTTAPPVQTPLPVIPHQKVVVNSPANADYQERFNPSALKDSALSTHKPIEVKGLGGKATIIHAQYNTPISMYSQDAIMDAIAGQAQAQGSDFSGSLPIKDLAVDSASPVYQAVIKSQNKPEDEADEWARRSSNLQSRSFRILAQMTGTEFMQDPDEEALRRSRERFETERNSPRFAKLRNWHHGLSAQILNVKS
Gene ID
Swiss Prot
SynonymsMFM4; ZASP; CMD1C; CMH24; CMPD3; LVNC3; CYPHER; LDB3Z1; LDB3Z4; ORACLE; PDLIM6; LDB3
Calculated MW77kDa
Observed MW50kDa/90kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHL-60, MCF7, U-937, Raji, BT-474, Mouse skeletal muscle, Rat skeletal muscle
Cellular locationCell projection, Cytoplasm, Z line, cytoskeleton, myofibril, perinuclear region, pseudopodium, sarcomere

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - LDB3 Rabbit pAb (A7462)}

    Western blot - LDB3 Rabbit pAb (A7462)

    Western blot analysis of various lysates using LDB3 Rabbit pAb (A7462) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Immunohistochemistry - LDB3 Rabbit pAb (A7462)}

    Immunohistochemistry - LDB3 Rabbit pAb (A7462)

    Immunohistochemistry analysis of paraffin-embedded Mouse stomach using LDB3 Rabbit pAb (A7462) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunofluorescence - LDB3 Rabbit pAb (A7462)}

    Immunofluorescence - LDB3 Rabbit pAb (A7462)

    Immunofluorescence analysis of paraffin-embedded human placenta using LDB3 Rabbit pAb (A7462) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - LDB3 Rabbit pAb (A7462)}

    Immunofluorescence - LDB3 Rabbit pAb (A7462)

    Immunofluorescence analysis of paraffin-embedded rat heart using LDB3 Rabbit pAb (A7462) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - LDB3 Rabbit pAb (A7462)}

    Immunofluorescence - LDB3 Rabbit pAb (A7462)

    Immunofluorescence analysis of paraffin-embedded mouse heart using LDB3 Rabbit pAb (A7462) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)