제품 > 항체 > 다중클론항체(pAb)

LEO1 Rabbit pAb (A8806)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - LEO1 Rabbit pAb (A8806)

Western blot analysis of various lysates using LEO1 Rabbit pAb (A8806) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - LEO1 Rabbit pAb (A8806)

Western blot analysis of various lysates using LEO1 Rabbit pAb (A8806) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - LEO1 Rabbit pAb (A8806)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using LEO1 Rabbit pAb (A8806) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - LEO1 Rabbit pAb (A8806)

Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using LEO1 Rabbit pAb (A8806) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - LEO1 Rabbit pAb (A8806)

Immunohistochemistry analysis of paraffin-embedded Mouse liver using LEO1 Rabbit pAb (A8806) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - LEO1 Rabbit pAb (A8806)

Immunofluorescence analysis of NIH/3T3 cells using LEO1 Rabbit pAb (A8806) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - LEO1 Rabbit pAb (A8806)

Immunofluorescence analysis of U2OS cells using LEO1 Rabbit pAb (A8806) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameLEO1 Rabbit pAb
Catalog No.A8806
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
LEO1, parafibromin (CDC73; MIM 607393), CTR9 (MIM 609366), and PAF1 (MIM 610506) form the PAF protein complex that associates with the RNA polymerase II subunit POLR2A (MIM 180660) and with a histone methyltransferase complex (Rozenblatt-Rosen et al., 2005 [PubMed 15632063]).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 340-570 of human LEO1 (NP_620147.1).
SequenceENGLPQDQQEEEPIPETRIEVEIPKVNTDLGNDLYFVKLPNFLSVEPRPFDPQYYEDEFEDEEMLDEEGRTRLKLKVENTIRWRIRRDEEGNEIKESNARIVKWSDGSMSLHLGNEVFDVYKAPLQGDHNHLFIRQGTGLQGQAVFKTKLTFRPHSTDSATHRKMTLSLADRCSKTQKIRILPMAGRDPECQRTEMIKKEEERLRASIRRESQQRRMREKQHQRGLSASYL
Gene ID
Swiss Prot
SynonymsRDL; LEO1
Calculated MW75kDa
Observed MW105kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHepG2, Jurkat, Mouse liver, Mouse brain, Rat brain
Cellular locationNucleus

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - LEO1 Rabbit pAb (A8806)}

    Western blot - LEO1 Rabbit pAb (A8806)

    Western blot analysis of various lysates using LEO1 Rabbit pAb (A8806) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Western blot - LEO1 Rabbit pAb (A8806)}

    Western blot - LEO1 Rabbit pAb (A8806)

    Western blot analysis of various lysates using LEO1 Rabbit pAb (A8806) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunohistochemistry - LEO1 Rabbit pAb (A8806)}

    Immunohistochemistry - LEO1 Rabbit pAb (A8806)

    Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using LEO1 Rabbit pAb (A8806) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - LEO1 Rabbit pAb (A8806)}

    Immunohistochemistry - LEO1 Rabbit pAb (A8806)

    Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using LEO1 Rabbit pAb (A8806) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - LEO1 Rabbit pAb (A8806)}

    Immunohistochemistry - LEO1 Rabbit pAb (A8806)

    Immunohistochemistry analysis of paraffin-embedded Mouse liver using LEO1 Rabbit pAb (A8806) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunofluorescence - LEO1 Rabbit pAb (A8806)}

    Immunofluorescence - LEO1 Rabbit pAb (A8806)

    Immunofluorescence analysis of NIH/3T3 cells using LEO1 Rabbit pAb (A8806) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - LEO1 Rabbit pAb (A8806)}

    Immunofluorescence - LEO1 Rabbit pAb (A8806)

    Immunofluorescence analysis of U2OS cells using LEO1 Rabbit pAb (A8806) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)