제품 > 항체 > 다중클론항체(pAb)

LGALS2 Rabbit pAb (A6645)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of extracts of Mouse stomach, using LGALS2 antibody (A6645) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - LGALS2 Rabbit pAb (A6645)

Immunofluorescence analysis of human colon carcinoma using LGALS2 Rabbit pAb (A6645) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of mouse Intestine using LGALS2 Rabbit pAb (A6645) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product name	LGALS2 Rabbit pAb
Catalog No.	A6645
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is a soluble beta-galactoside binding lectin. The encoded protein is found as a homodimer and can bind to lymphotoxin-alpha. A single nucleotide polymorphism in an intron of this gene can alter the transcriptional level of the protein, with a resultant increased risk of myocardial infarction.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-74 of human LGALS2 (NP_006489.1).
Sequence	MTGELEVKNMDMKPGSTLKITGSIADGTDGFVINLGQGTDKLNLHFNPRFSESTIVCNSLDGSNWGQEQREDHL
Gene ID
Swiss Prot
Synonyms	HL14
Calculated MW	15kDa
Observed MW	15kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:100 - 1:500 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	Mouse stomach
Cellular location	cytoplasm
Customer validation	WB(Homo sapiens)

Documents

Certificate of Compliance

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Research Area

LGALS2 Rabbit pAb images

Western blot - LGALS2 Rabbit pAb (A6645)

Immunofluorescence - LGALS2 Rabbit pAb (A6645)

Immunofluorescence analysis of human colon carcinoma using LGALS2 Rabbit pAb (A6645) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence - LGALS2 Rabbit pAb (A6645)

Immunofluorescence analysis of mouse Intestine using LGALS2 Rabbit pAb (A6645) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.