제품 > 항체 > 다중클론항체(pAb)

LHX3 Rabbit pAb (A17015)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - LHX3 Rabbit pAb (A17015)

Western blot analysis of various lysates using LHX3 Rabbit pAb (A17015) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameLHX3 Rabbit pAb
Catalog No.A17015
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of a large family of proteins which carry the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein is a transcription factor that is required for pituitary development and motor neuron specification. Mutations in this gene cause combined pituitary hormone deficiency 3. Alternative splicing results in multiple transcript variants encoding different isoforms.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human LHX3 (NP_055379.1).
SequenceLEHGGLAGPEQYRELRPGSPYGVPPSPAAPQSLPGPQPLLSSLVYPDTSLGLVPSGAPGGPPPMRVLAGNGPSSDLSTGSSGGYPDFPASPASWLDEVDHAQF
Gene ID
Swiss Prot
SynonymsLIM3; CPHD3; M2-LHX3; LHX3
Calculated MW43kDa
Observed MW43kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHepG2, SH-SY5Y, U-87MG, U-251MG, Mouse brain, Rat brain
Cellular locationnucleus
ABclonal:Western blot - LHX3 Rabbit pAb (A17015)}

Western blot - LHX3 Rabbit pAb (A17015)

Western blot analysis of various lysates using LHX3 Rabbit pAb (A17015) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)