제품 > 항체 > 다중클론항체(pAb)

LIMD1 Rabbit pAb (A17585)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - LIMD1 Rabbit pAb (A17585)

Western blot analysis of various lysates using LIMD1 Rabbit pAb (A17585) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product nameLIMD1 Rabbit pAb
Catalog No.A17585
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable transcription corepressor activity. Involved in several processes, including negative regulation of hippo signaling; regulation of gene expression; and response to hypoxia. Acts upstream of or within P-body assembly and gene silencing by miRNA. Located in several cellular components, including P-body; adherens junction; and focal adhesion. Part of RISC complex.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human LIMD1 (NP_055055.1).
SequenceMDKYDDLGLEASKFIEDLNMYEASKDGLFRVDKGAGNNPEFEETRRVFATKMAKIHLQQQQQQLLQEETLPRGSRGPVNGGGRLGPQARWEVVGSKLTVDGAAKPPLAASTGAPGAVTTLAAGQPPYPPQEQRSRPYLHGTRHGSQDCGSRESLATSEMSAFHQPGPCEDPSCLTHGDYYDNLSLASPKWGDKPGVSPSI
Gene ID
Swiss Prot
SynonymsLIMD1
Calculated MW72kDa
Observed MW72kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMCF7, HeLa, HepG2, Mouse lung, Mouse heart
Cellular locationcytoplasm, cytosol, focal adhesion, nucleoplasm, nucleus, P-body, plasma membrane

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    ABclonal:Western blot - LIMD1 Rabbit pAb (A17585)}

    Western blot - LIMD1 Rabbit pAb (A17585)

    Western blot analysis of various lysates using LIMD1 Rabbit pAb (A17585) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)