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LIMS1 Rabbit pAb (A6384)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - LIMS1 Rabbit pAb (A6384)

Western blot analysis of extracts of HepG2 cells, using LIMS1 antibody (A6384) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

ABclonal:Immunofluorescence - LIMS1 Rabbit pAb (A6384)

Immunofluorescence analysis of MCF7 cells using LIMS1 antibody (A6384). Blue: DAPI for nuclear staining.

Overview

Product nameLIMS1 Rabbit pAb
Catalog No.A6384
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is an adaptor protein which contains five LIM domains, or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that this protein may play a role in integrin-mediated cell adhesion or spreading. Several transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-325 of human LIMS1 (NP_001180417.1).
SequenceMANALASATCERCKGGFAPAEKIVNSNGELYHEQCFVCAQCFQQFPEGLFYEFEGRKYCEHDFQMLFAPCCHQCGEFIIGRVIKAMNNSWHPECFRCDLCQEVLADIGFVKNAGRHLCRPCHNREKARGLGKYICQKCHAIIDEQPLIFKNDPYHPDHFNCANCGKELTADARELKGELYCLPCHDKMGVPICGACRRPIEGRVVNAMGKQWHVEHFVCAKCEKPFLGHRHYERKGLAYCETHYNQLFGDVCFHCNRVIEGDVVSALNKAWCVNCFACSTCNTKLTLKNKFVEFDMKPVCKKCYEKFPLELKKRLKKLAETLGRK
Gene ID
Swiss Prot
SynonymsPINCH; PINCH1; PINCH-1
Calculated MW37kDa
Observed MW40kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHepG2
Cellular locationCell junction, Cell membrane, Cytoplasmic side, Peripheral membrane protein, focal adhesion

Documents

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    ABclonal:Western blot - LIMS1 Rabbit pAb (A6384)}

    Western blot - LIMS1 Rabbit pAb (A6384)

    Western blot analysis of extracts of HepG2 cells, using LIMS1 antibody (A6384) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 90s.
    ABclonal:Immunofluorescence - LIMS1 Rabbit pAb (A6384)}

    Immunofluorescence - LIMS1 Rabbit pAb (A6384)

    Immunofluorescence analysis of MCF7 cells using LIMS1 antibody (A6384). Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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