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LIN28B Rabbit mAb (A9524)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - LIN28B Rabbit mAb (A9524)

Western blot analysis of various lysates using LIN28B Rabbit mAb (A9524) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

ABclonal:Immunohistochemistry - LIN28B Rabbit mAb (A9524)

Immunohistochemistry analysis of paraffin-embedded Human placenta using LIN28B Rabbit mAb (A9524) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Overview

Product nameLIN28B Rabbit mAb
Catalog No.A9524
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1616
The protein encoded by this gene belongs to the lin-28 family, which is characterized by the presence of a cold-shock domain and a pair of CCHC zinc finger domains. This gene is highly expressed in testis, fetal liver, placenta, and in primary human tumors and cancer cell lines. It is negatively regulated by microRNAs that target sites in the 3' UTR, and overexpression of this gene in primary tumors is linked to the repression of let-7 family of microRNAs and derepression of let-7 targets, which facilitates cellular transformation.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-163 of human LIN28B (Q6ZN17).
SequenceMAEGGASKGGGEEPGKLPEPAEEESQVLRGTGHCKWFNVRMGFGFISMINREGSPLDIPVDVFVHQSKLFMEGFRSLKEGEPVEFTFKKSSKGLESIRVTGPGGSPCLGSERRPKGKTLQKRKPKGDRCYNCGGLDHHAKECSLPPQPKKCHYCQSIMHMVAN
Gene ID
Swiss Prot
SynonymsCSDD2; LIN28B
Calculated MW27kDa
Observed MW21kDa/32kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samples293T, Hep G2
Cellular locationCytoplasm, Nucleus, nucleolus

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ABclonal:Western blot - LIN28B Rabbit mAb (A9524)}

Western blot - LIN28B Rabbit mAb (A9524)

Western blot analysis of various lysates using LIN28B Rabbit mAb (A9524) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
ABclonal:Immunohistochemistry - LIN28B Rabbit mAb (A9524)}

Immunohistochemistry - LIN28B Rabbit mAb (A9524)

Immunohistochemistry analysis of paraffin-embedded Human placenta using LIN28B Rabbit mAb (A9524) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)