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LMAN1 Rabbit mAb (A4941)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - LMAN1 Rabbit mAb (A4941)

Western blot analysis of various lysates using LMAN1 Rabbit mAb (A4941) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - LMAN1 Rabbit mAb (A4941)

Immunohistochemistry analysis of paraffin-embedded Rat brain using LMAN1 Rabbit mAb (A4941) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - LMAN1 Rabbit mAb (A4941)

Immunohistochemistry analysis of paraffin-embedded Human appendix using LMAN1 Rabbit mAb (A4941) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - LMAN1 Rabbit mAb (A4941)

Immunohistochemistry analysis of paraffin-embedded Human placenta using LMAN1 Rabbit mAb (A4941) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Overview

Product nameLMAN1 Rabbit mAb
Catalog No.A4941
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1978
The protein encoded by this gene is a membrane mannose-specific lectin that cycles between the endoplasmic reticulum, endoplasmic reticulum-Golgi intermediate compartment, and cis-Golgi, functioning as a cargo receptor for glycoprotein transport. The protein has an N-terminal signal sequence, a calcium-dependent and pH-sensitive carbohydrate recognition domain, a stalk region that functions in oligomerization, a transmembrane domain, and a short cytoplasmic domain required for organelle targeting. Allelic variants of this gene are associated with the autosomal recessive disorder combined factor V-factor VIII deficiency.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human LMAN1 (P49257).
SequenceMAGSRQRGLRARVRPLFCALLLSLGRFVRGDGVGGDPAVALPHRRFEYKYSFKGPHLVQSDGTVPFWAHAGNAIPSSDQIRVAPSLKSQRGSVWTKTKAA
Gene ID
Swiss Prot
SynonymsMR60; gp58; F5F8D; FMFD1; MCFD1; ERGIC53; ERGIC-53; LMAN1
Calculated MW54kDa
Observed MW58kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa, 293T, NCI-H460, Mouse liver, Mouse brain, Mouse heart, Rat lung, Rat liver, Rat kidney
Cellular locationEndoplasmic reticulum membrane, Endoplasmic reticulum-Golgi intermediate compartment membrane, Golgi apparatus membrane, Single-pass membrane protein, Single-pass type I membrane protein

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - LMAN1 Rabbit mAb (A4941)}

    Western blot - LMAN1 Rabbit mAb (A4941)

    Western blot analysis of various lysates using LMAN1 Rabbit mAb (A4941) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Immunohistochemistry - LMAN1 Rabbit mAb (A4941)}

    Immunohistochemistry - LMAN1 Rabbit mAb (A4941)

    Immunohistochemistry analysis of paraffin-embedded Rat brain using LMAN1 Rabbit mAb (A4941) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - LMAN1 Rabbit mAb (A4941)}

    Immunohistochemistry - LMAN1 Rabbit mAb (A4941)

    Immunohistochemistry analysis of paraffin-embedded Human appendix using LMAN1 Rabbit mAb (A4941) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - LMAN1 Rabbit mAb (A4941)}

    Immunohistochemistry - LMAN1 Rabbit mAb (A4941)

    Immunohistochemistry analysis of paraffin-embedded Human placenta using LMAN1 Rabbit mAb (A4941) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.

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