제품 > 항체 > 다중클론항체(pAb)

LMAN2L Rabbit pAb (A18505)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - LMAN2L Rabbit pAb (A18505)

Western blot analysis of extracts of HeLa cells, using LMAN2L antibody (A18505) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunofluorescence - LMAN2L Rabbit pAb (A18505)

Immunofluorescence analysis of C6 cells using LMAN2L Rabbit pAb (A18505) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - LMAN2L Rabbit pAb (A18505)

Immunofluorescence analysis of NIH/3T3 cells using LMAN2L Rabbit pAb (A18505) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - LMAN2L Rabbit pAb (A18505)

Immunofluorescence analysis of U2OS cells using LMAN2L Rabbit pAb (A18505) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameLMAN2L Rabbit pAb
Catalog No.A18505
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein belonging to the L-type lectin group of type 1 membrane proteins, which function in the mammalian early secretory pathway. These proteins contain luminal carbohydrate recognition domains, which display homology to leguminous lectins. Unlike other proteins of the group, which cycle in the early secretory pathway and are predominantly associated with post endoplasmic reticulum membranes, the protein encoded by this gene is a non-cycling resident protein of the ER, where it functions as a cargo receptor for glycoproteins. It is proposed to regulate exchange of folded proteins for transport to the Golgi and exchange of misfolded glycoproteins for transport to the ubiquitin-proteasome pathway.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 189-348 of human LMAN2L (NP_110432.1).
SequenceERDGRPTELGGCTAIVRNLHYDTFLVIRYVKRHLTIMMDIDGKHEWRDCIEVPGVRLPRGYYFGTSSITGDLSDNHDVISLKLFELTVERTPEEEKLHRDVFLPSVDNMKLPEMTAPLPPLSGLALFLIVFFSLVFSVFAIVIGIILYNKWQEQSRKRFY
Gene ID
Swiss Prot
SynonymsVIPL; MRD69; MRT52
Calculated MW40kDa
Observed MW40kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHeLa
Cellular locationCOPII-coated ER to Golgi transport vesicle, endoplasmic reticulum-Golgi intermediate compartment, Golgi apparatus

Documents

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    ABclonal:Western blot - LMAN2L Rabbit pAb (A18505)}

    Western blot - LMAN2L Rabbit pAb (A18505)

    Western blot analysis of extracts of HeLa cells, using LMAN2L antibody (A18505) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 3s.
    ABclonal:Immunofluorescence - LMAN2L Rabbit pAb (A18505)}

    Immunofluorescence - LMAN2L Rabbit pAb (A18505)

    Immunofluorescence analysis of C6 cells using LMAN2L Rabbit pAb (A18505) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - LMAN2L Rabbit pAb (A18505)}

    Immunofluorescence - LMAN2L Rabbit pAb (A18505)

    Immunofluorescence analysis of NIH/3T3 cells using LMAN2L Rabbit pAb (A18505) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - LMAN2L Rabbit pAb (A18505)}

    Immunofluorescence - LMAN2L Rabbit pAb (A18505)

    Immunofluorescence analysis of U2OS cells using LMAN2L Rabbit pAb (A18505) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)