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L-Plastin/LCP1 Rabbit pAb (A5561)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - L-Plastin/LCP1 Rabbit pAb (A5561)

Western blot analysis of various lysates using L-Plastin/LCP1 Rabbit pAb (A5561) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunoprecipitation - L-Plastin/LCP1 Rabbit pAb (A5561)

Immunoprecipitation analysis of 150 μg extracts of Jurkat cells using 3 μg L-Plastin/LCP1 antibody (A5561). Western blot was performed from the immunoprecipitate using L-Plastin/LCP1 antibody (A5561) at a dilution of 1:1000.

Overview

Product nameL-Plastin/LCP1 Rabbit pAb
Catalog No.A5561
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). However, L-plastin has been found in many types of malignant human cells of non-hemopoietic origin suggesting that its expression is induced accompanying tumorigenesis in solid tissues.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human L-Plastin/LCP1 (NP_002289.2).
SequenceMARGSVSDEEMMELREAFAKVDTDGNGYISFNELNDLFKAACLPLPGYRVREITENLMATGDLDQDGRISFDEFIKIFHGLKSTDVAKTFRKAINKKEGICAIGGTSEQSSVGTQHSYSEEEKYAFVNWINKALENDPDCRHVIPMNPNTNDLFNAVGDGIVLCKMINLSVPDTIDERTINKKKLTPFTIQENLNLALNSASAIGCHVVNIGAEDLKEGKPYLVLGLLWQVIKIGLFADIELSRNEALIALLREGESLEDLMKLSPEELL
Gene ID
Swiss Prot
SynonymsLPL; CP64; PLS2; LC64P; HEL-S-37; L-PLASTIN; L-Plastin/LCP1
Calculated MW70kDa
Observed MW70kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunoprecipitation    
Positive samplesRaji, K-562, DU145, Jurkat, Mouse thymus, Mouse spleen
Cellular locationCell junction, Cell projection, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, cytoskeleton, ruffle membrane
Customer validation

WB(Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - L-Plastin/LCP1 Rabbit pAb (A5561)}

Western blot - L-Plastin/LCP1 Rabbit pAb (A5561)

Western blot analysis of various lysates using L-Plastin/LCP1 Rabbit pAb (A5561) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunoprecipitation - L-Plastin/LCP1 Rabbit pAb (A5561)}

Immunoprecipitation - L-Plastin/LCP1 Rabbit pAb (A5561)

Immunoprecipitation analysis of 150 μg extracts of Jurkat cells using 3 μg L-Plastin/LCP1 antibody (A5561). Western blot was performed from the immunoprecipitate using L-Plastin/LCP1 antibody (A5561) at a dilution of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (1)

Secondary Antibodies (26)