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LRRC4C Rabbit pAb (A15491)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Rat

ABclonal:Western blot - LRRC4C Rabbit pAb (A15491)

Western blot analysis of lysates from rat eye, using LRRC4C Rabbit pAb (A15491) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product nameLRRC4C Rabbit pAb
Catalog No.A15491
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
NGL1 is a specific binding partner for netrin G1 (NTNG1; MIM 608818), which is a member of the netrin family of axon guidance molecules (Lin et al., 2003 [PubMed 14595443]).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 450-520 of human LRRC4C (NP_065980.1).
SequencePFSYFSTVTVETMEPSQDEARTTDNNVGPTPVVDWETTNVTTSLTPQSTRSTEKTFTIPVTDINSGIPGID
Gene ID
Swiss Prot
SynonymsNGL1; NGL-1; LRRC4C
Calculated MW72kDa
Observed MW72kDa
ReactivityRat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:200 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesRat eye
Cellular locationCell junction, Single-pass type I membrane protein, postsynaptic cell membrane, synapse
ABclonal:Western blot - LRRC4C Rabbit pAb (A15491)}

Western blot - LRRC4C Rabbit pAb (A15491)

Western blot analysis of lysates from rat eye, using LRRC4C Rabbit pAb (A15491) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)